留言板
- [2]袁顺波
- 尊敬的科学网博客用户：
您好，诚恳地邀请您参与“科学网博客用户持续使用的影响因素”问卷调查，网址是：
http://www.sojump.com/jq/2250398.aspx，本次调查需要3-5分钟的时间，非常感谢！
- [1]明振华
- 您好，感谢您上次帮我找到了文献，现在我还有一篇文献没有找到，但是科学网的求助系统似乎除了问题，所以只能以留言的方式向您求助，希望您方便之余能再次帮忙。
文献信息：
标 题：Selective induction of endothelial P2Y6 nucleotide receptor promotes vascular inflammasome
作 者：Ann-Kathrin Riegel1,2, Marion Faigle1, Stephanie Zug1, Peter Rosenberger3, Bernard Robaye4, Jean-Marie Boeynaems5, Marco Idzko6, and Holger K. Eltzschig2
期刊名称：
期卷页： 第卷 第期 页
摘 要：During a systemic inflammatory response endothelial-expressed surface molecules have been strongly implicated in orchestrating immune responses. Previous studies have shown enhanced extracellular nucleotide release during acute inflammatory conditions. Therefore, we hypothesized that endothelial nucleotide receptors could play a role in vascular inflammation. To address this hypothesis, we performed screening experiments and exposed human microvascular endothelia to inflammatory stimuli, followed by measurements of P2Y or P2X transcriptional responses. These studies showed a selective induction of the P2Y6 receptor (> 4-fold at 24 hours). Moreover, studies that used real-time reverse transcription–polymerase chain reaction, Western blot analysis, or immunofluorescence confirmed time- and dose-dependent induction of P2Y6 with tumor necrosis factor α or Lipopolysaccharide (LPS) stimulation in vitro and in vivo. Studies that used MRS 2578 as P2Y6 receptor antagonist showed attenuated nuclear factor κB reporter activity and proinflammatory gene expression in human microvascular endothelial cells in vitro. Moreover, pharmacologic or genetic in vivo studies showed attenuated inflammatory responses in P2Y6−/− mice or after P2Y6 antagonist treatment during LPS-induced vascular inflammation. These studies show an important contribution of P2Y6 signaling in enhancing vascular inflammation during systemic LPS challenge and implicate the P2Y6 receptor as a therapeutic target during systemic inflammatory responses.
文献频道链接：http://doc.sciencenet.cn/DocInfo.aspx?id=8302
原文链接：http://bloodjournal.hematologylibrary.org/content/117/8/2548.abstract - 我的回复(2012-3-1 14:26)：给你下到了，但是找不到你的邮箱
