Protoplast culture of rice ( Oryza sativa L.) using media solidified with agarose J. A. Thompson, R. Abdullah * and E. C. Cocking Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, Nottingham NG7 2RD, U.K. Received 17 February 1986;&n ...
Surface Sterilization of Plant Explants and Orchid Seed To avoid bacterial and fungal growth, which is detrimental to culture growth, explants are surface-sterilized before they are used to establish in vitro axenic cultures. The most common disinfectants are listed below with the concentrat ...
Plant tissue culture media are generally sterilized by autoclaving at 121 C and 1.05 kg/cm2 (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel. The minimum times required for sterilization of different volumes of medium are listed below. It is advis ...
Phytagel is an agar substitute produced from a bacterial substrate composed of glucuronic acid葡萄糖醛酸, rhamnose 鼠李糖 and glucose. It produces a clear, colorless, high-strength gel, which aids in detection of microbial contamination. Phytagel provides an economical alternative to agar as a gell ...
Plant barcodes 18S rDNA (1855 bp), rbcL (1428 bp), and atpB (1450 bp) representing a total of 4733 bp Plastid rbcL: the large subunit of Rubisco (ribulose bisphosphate carboxylase) 1,5- 二磷酸核酮糖羧化酶 / 加氧酶大亚基 ribulose-1,5-bisp ...
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