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很好的一本书 Agrobacterium: From Biology to Biotechnology
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Agrobacterium: From Biology to Biotechnology
Edited by
Tzvi Tzfira
Department of Molecular, Cellular and Developmental Biology
University of Michigan
Ann Arbor, MI, USA
and
Vitaly Citovsky
Department of Biochemistry and Cell Biology
State University of New York
Stony Brook, NY, USA
steps in transformation:
1. Agrobacterium attachment to the plant cell;
2. Transfer of T-strands and virulence effector proteins through the plant plasma membrane into the plant cell;
3. T-complex and super-T-complex formation and subsequent cytoplasmic trafficking;
4. Nuclear targeting;
5. Targeting of the super-Tcomplex to chromatin;
6. Removal of proteins from the super-T-complex prior to T-DNA integration;
7. T-DNA integration into the plant genome;
8. Transgene expression.
https://wap.sciencenet.cn/blog-38240-317343.html
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