TickingClock的个人博客分享 http://blog.sciencenet.cn/u/TickingClock

博文

Plant Physiology:生长素信号感知下游一个胁迫相关基因组成的调控网络介导下胚轴伸长调控

已有 1740 次阅读 2021-6-16 15:07 |个人分类:每日摘要|系统分类:论文交流

A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception

第一作者Adeline Rigal

第一单位瑞典农业科学大学

通讯作者Stéphanie Robert


 Abstract 


背景回顾The plant hormone auxin, a master coordinator of development, regulates hypocotyl elongation during seedling growth. 


研究基础:We previously identified the synthetic molecule RubNeddin 1 (RN1), which induces degradation of the AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors INDOLE-3-ACETIC ACID INDUCIBLE3 (IAA3) and IAA7 in planta and strongly promotes hypocotyl elongation


主要研究:In the present study, we show that despite the structural similarity of RN1 to the synthetic auxin 2,4-dichlorophenoxyacetic-acid (2,4-D), direct treatments with these compounds in Arabidopsis (Arabidopsis thaliana) result in distinct effects, possibly due to enhanced uptake of RN1 and low-level, chronic release of 2,4-D from RN1 in planta.


结果1-RN1与下胚轴伸长:We confirm RN1-induced hypocotyl elongation occurs via specific TRANSPORT INHIBITOR RESISTANT1 (TIR1)/AUXIN SIGNALING F-BOX (AFB) receptor-mediated auxin signaling involving TIR1AFB2 and AFB5


结果2-下游基因:Using a transcriptome profiling strategy and candidate gene approach, we identify the genes ZINC FINGER OF ARABIDOPSIS THALIANA10 (ZAT10), ARABIDOPSIS TOXICOS EN LEVADURA31 (ATL31) and WRKY DNA-BINDING PROTEIN33 (WRKY33) as being rapidly up-regulated by RN1, despite being down-regulated by 2,4-D treatment. RN1-induced expression of these genes also occurs via TIR1/AFB-mediated auxin signaling. 


结果3-RN1作用机制Our results suggest both hypocotyl elongation and transcription of these genes are induced by RN1 via the promoted degradation of the AUX/IAA transcriptional repressor IAA7


结果4-胁迫相关:Moreover, these three genes, which are known to be stress-related, act in an inter-dependent transcriptional regulatory network controlling hypocotyl elongation. 


结论:Together, our results suggest ZAT10ATL31 and WRKY33 take part in a common gene network regulating hypocotyl elongation in Arabidopsis downstream of a selective auxin perception module likely involving TIR1, AFB2 and AFB5 and inducing the degradation of IAA7.


 摘 要 


植物激素生长素是发育的重要协调因子,在植株生长过程中调控下胚轴的伸长。作者先前鉴定了一个合成分子RN1,其能够诱导AUX/IAA转录抑制子IAA3和IAA7的降解,从而极大地促进了下胚轴的伸长。本文中,作者发现尽管RN1与合成的生长素2,4-D在结构上相似,但用这些化合物直接处理拟南芥会导致明显不同的表型,这可能是由于植物对RN1吸收更强,以及植物中来自RN1的低水平、慢性释放的2,4-D所致。作者进一步发现RN1诱导的下胚轴伸长主要是通过TIR1/AFB受体介导的生长素信号转导通路来发挥作用的,该通路涉及到了TIR1、AFB2和AFB5。利用转录组图谱和候选基因鉴定的方法,作者鉴定到了ZAT10ATL31WRKY33基因能够被RN1刺激,快速上调表达,但是2,4-D处理会导致这两个基因的下调表达。RN1诱导的基因表达同样也是通过TIR1/AFB介导的生长素信号转导通路。本文的结果揭示了RN1诱导的下胚轴伸长和基因转录调控,均是通过促进AUX/IAA转录抑制子IAA7的降解来发挥功能的。此外,这三个基因的功能均与胁迫相关,在相互依赖的转录调控网络中发挥作用,控制下胚轴的伸长。综上,本文的结果表明,ZAT10ATL31WRKY33参与了一个共同的基因网络,调控拟南芥下胚轴的伸长,作用于一个选择性生长素感知模块的下游,而该模块可能涉及TIR1、AFB2和AFB5,并诱导IAA7的降解。


 通讯作者 

** Stéphanie Robert **


个人简介:

2005年,巴黎第十一大学,博士;

2005-2007年,美国加州大学河滨分校博后;

2007-2010年,比利时根特大学,博后


研究方向揭示植物发育的调控机制,重点研究细胞壁、植物激素、膜内转运和分子信号在控制细胞形态和组织形态发生中的作用。


doi: https://doi.org/10.1093/plphys/kiab269


Journal: Plant Physiology

Published date: June 12, 2021



https://wap.sciencenet.cn/blog-3158122-1291452.html

上一篇:Nature Plants:银杏高质量基因组
下一篇:Nature Communications:喜树基因组揭示喜树碱生物合成的演化
收藏 IP: 202.119.212.*| 热度|

0

该博文允许注册用户评论 请点击登录 评论 (0 个评论)

数据加载中...

Archiver|手机版|科学网 ( 京ICP备07017567号-12 )

GMT+8, 2024-3-29 05:31

Powered by ScienceNet.cn

Copyright © 2007- 中国科学报社

返回顶部