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PNAS:拟南芥根组织中创伤诱导的细胞膨压变化和局部生长素信号协调根的恢复性分裂

已有 1115 次阅读 2020-6-21 10:11 |个人分类:每日摘要|系统分类:论文交流

Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots

第一作者Lukas Hoermayer

第一单位奥地利科学技术学院

通讯作者Jiří Friml


 Abstract 

Abstract

背景回顾+问题Wound healing in plant tissues, consisting of rigid cell wall-encapsulated cells, represents a considerable challenge and occurs through largely unknown mechanisms distinct from those in animals. Owing to their inability to migrate, plant cells rely on targeted cell division and expansion to regenerate wounds. Strict coordination of these wound-induced responses is essential to ensure efficient, spatially restricted wound healing. 


技术基础Single-cell tracking by live imaging allowed us to gain mechanistic insight into the wound perception and coordination of wound responses after laser-based wounding in Arabidopsis root.


结果发现:We revealed a crucial contribution of the collapse of damaged cells in wound perception and detected an auxin increase specific to cells immediately adjacent to the wound. This localized auxin increase balances wound-induced cell expansion and restorative division rates in a dose-dependent manner, leading to tumorous overproliferation when the canonical TIR1 auxin signaling is disrupted. Auxin and wound-induced turgor pressure changes together also spatially define the activation of key components of regeneration, such as the transcription regulator ERF115.


结论:Our observations suggest that the wound signaling involves the sensing of collapse of damaged cells and a local auxin signaling activation to coordinate the downstream transcriptional responses in the immediate wound vicinity.


 摘  要 

摘  要

植物组织的创伤自愈,形成由硬质细胞壁包裹着的细胞,代表了植物科学研究领域的一个重要挑战,其潜在的分子调控机制与动物中的自愈机制不同,且在很大程度上都处于未知状态。由于其无法迁移的特性,植物细胞必须依赖于靶向细胞分裂和扩张来再生创伤组织。伤口诱导响应之间的严密协调保证了高效地、组织部位特异性地创伤自愈。本文中,作者通过实时成像进行单细胞追踪,研究了拟南芥根组织中激光烧蚀后的创伤感知以及创伤响应之间的协调。作者发现了受损伤细胞的瓦解对于创伤感知具有非常关键的作用;另外,细胞受损伤之后,其毗邻细胞中立马检测到了生长素的增加。这种局部生长素通过剂量依赖性的方式增强了创伤诱导细胞扩张与恢复性分裂速率之间的平衡,在经典TIR1生长素信号转导被扰乱后导致肿瘤式的过度增殖。生长素和创伤诱导的膨压变化同时作用于局部再生关键组份的激活,比如说转录调控因子ERF115。本研究的发现揭示了植物中创伤信号转导涉及了受损伤细胞瓦解的感知以及局部生长素信号的激活,从而来协调伤口邻近处即时的下游转录响应。


 通讯作者 

**Jiří Friml**


个人简介:

1995年,马萨里克大学,化学学士;

1997年,马萨里克大学,生物化学学士;

2000年,科隆大学,生物学博士;

2002年,马萨里克大学,生物化学博士。


研究方向:

生长素的极性运输;

细胞极性;

细胞内吞与再循环;

信号转导的非转录机制。


doi: 10.1073/pnas.2003346117


Journal: PNAS

First Published: June 15, 2020


p.s. Jiří Friml往期文章链接



https://wap.sciencenet.cn/blog-3158122-1238730.html

上一篇:Plant Communications:植物RALF-FERONIA信号综述~植物免疫响应与细胞生长
下一篇:The EMBO Journal:植物E3连接酶调控感应NLR免疫受体的内稳态

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