||
致敬韩春雨老师-“不鸣则已,一鸣惊人”在Nature Biotech发表重 精选
http://blog.sciencenet.cn/blog-300826-975762.html
5月2日论文发表后受到国际学界的关注,我们会继续跟踪国际反响和评论。
Altmetric替代计量得分 55分 https://www.altmetric.com/details/7114638
Country | Count | As % |
---|---|---|
United States 美国 | 16 | 20% |
United Kingdom 英国 | 9 | 11% |
Spain 西班牙 | 5 | 6% |
Germany 德国 | 3 | 4% |
Switzerland 瑞士 | 2 | 2% |
Japan 日本 | 2 | 2% |
Brazil 巴西 | 1 | 1% |
Colombia 哥伦比亚 | 1 | 1% |
Argentina 阿根廷 | 1 | 1% |
Other | 2 | 2% |
Unknown | 39 | 48% |
Type | Count | As % |
---|---|---|
Members of the public 公众 | 46 | 57% |
Scientists 科学家 | 34 | 42% |
Science communicators (journalists, bloggers, editors) 科学媒体人 | 1 | 1% |
韩春雨老师近照(来源:河北科技大学微信号)
DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.
1Department of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
2Department of Physiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ~24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo-gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9, and preliminary characterization suggests a low tolerance to guide-target mismatches and high efficiency in editing (G+C)-rich genomic targets.
Gao F . Shen XZ . Jiang F . Wu Y . Han C .
Department of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
来源: Nat Biotechnol ( P 1087-0156 E 1546-1696 ) IF:41.514 H指数:251 年: 2016
PMID: 27136078 [Pubmed] 生物工程与应用微生物,1区
Archiver|手机版|科学网 ( 京ICP备07017567号-12 )
GMT+8, 2024-10-15 08:02
Powered by ScienceNet.cn
Copyright © 2007- 中国科学报社