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韩春雨基因编辑研究的战略与战术

已有 2773 次阅读 2016-10-15 09:12 |个人分类:今日热点|系统分类:观点评述

第565次香山科学会议“基因编辑技术的研究与应用”学术讨论会

主题评述报告：

基因编辑：中国的困境、机遇与挑战

中心议题评述报告：

1. 基于Ago系统的基因编辑

2. 靶向核酸酶基因编辑在人类多能性干细胞和小鼠模型制备中的应用

http://www.meeting.edu.cn/meeting/subject/meetingAction-71779!detail.action

许培扬基因编辑（Gene Editing）2017年成为美国PUBMED系统正式主题词

http://blog.sciencenet.cn/home.php?mod=space&uid=280034&do=blog&id=1008705

基因编辑国际研究文献5985篇分布如下： http://blog.sciencenet.cn/blog-280034-1008791.html

数据来源 http://www.pubmedplus.cn/

基因编辑国际研究期刊影响因子大于20、H指数大于130的论文296篇分布如下：

http://blog.sciencenet.cn/blog-280034-1008807.html

中国大陆科学家取得的重大科研成果：

Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo. 百度翻译

Yin H . Song CQ . Dorkin JR . Zhu LJ . Li Y . Wu Q . Park A . Yang J . Suresh S . Bizhanova A . Gupta A . Bolukbasi MF . Walsh S . Bogorad RL . Gao G . Weng Z . Dong Y . Koteliansky V . Wolfe SA . Langer R . Xue W . Anderson DG .
David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
来源: Nat Biotechnol ( P 1087-0156 E 1546-1696 ) IF:43.113(2015) H指数:251 年: 2016 卷: 34 期: 3 页: 328-33
PMID: 26829318 [Pubmed] 生物工程与应用微生物,1区

CRISPR Inversion of CTCF Sites Alters Genome Topology and Enhancer/Promoter Function. 百度翻译

Guo Y . Xu Q . Canzio D . Shou J . Li J . Gorkin DU . Jung I . Wu H . Zhai Y . Tang Y . Lu Y . Wu Y . Jia Z . Li W . Zhang MQ . Ren B . Krainer AR . Maniatis T . Wu Q .
Center for Comparative Biomedicine, MOE Key Laboratory of Systems Biomedicine, Institute of Systems Biomedicine, Collaborative Innovation Center of Systems Biomedicine, Shanghai Jiao Tong University (SJTU), Shanghai 200240, China; State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, SJTU, Shanghai?200240, China; Key Laboratory for the Genetics of Developm
来源: Cell ( P 0092-8674 E 1097-4172 ) IF:28.71(2015) H指数:503 年: 2015 卷: 162 期: 4 页: 900-10
PMID: 26276636 [Pubmed] 生化与分子生物学,1区

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Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. 百度翻译

Chen Y . Cao J . Xiong M . Petersen AJ . Dong Y . Tao Y . Huang CT . Du Z . Zhang SC .
Waisman Center, University of Wisconsin, Madison, WI 53705, USA. Electronic address: yuejunchenphd@gmail.com.
来源: Cell Stem Cell ( P 1934-5909 E 1875-9777 ) IF:22.387(2015) H指数:85 年: 2015 卷: 17 期: 2 页: 233-44
PMID: 26145478 [Pubmed] 细胞生物学,1区

CRISPR germline engineering--the community speaks. 百度翻译

Bosley KS . Botchan M . Bredenoord AL . Carroll D . Charo RA . Charpentier E . Cohen R . Corn J . Doudna J . Feng G . Greely HT . Isasi R . Ji W . Kim JS . Knoppers B . Lanphier E . Li J . Lovell-Badge R . Martin GS . Moreno J . Naldini L . Pera M . Perry AC . Venter JC . Zhang F . Zhou Q .
Editas Medicine, Cambridge, Massachusetts, USA.
来源: Nat Biotechnol ( P 1087-0156 E 1546-1696 ) IF:43.113(2015) H指数:251 年: 2015 卷: 33 期: 5 页: 478-86
PMID: 25965754 [Pubmed] 生物工程与应用微生物,1区

Selective elimination of mitochondrial mutations in the germline by genome editing. 百度翻译

Reddy P . Ocampo A . Suzuki K . Luo J . Bacman SR . Williams SL . Sugawara A . Okamura D . Tsunekawa Y . Wu J . Lam D . Xiong X . Montserrat N . Esteban CR . Liu GH . Sancho-Martinez I . Manau D . Civico S . Cardellach F . Del Mar O'Callaghan M . Campistol J . Zhao H . Campistol JM . Moraes CT . Izpisua Belmonte JC .
Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
来源: Cell ( P 0092-8674 E 1097-4172 ) IF:28.71(2015) H指数:503 年: 2015 卷: 161 期: 3 页: 459-69
PMID: 25910206 [Pubmed] 生化与分子生物学,1区

Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation. 百度翻译

Yin Y . Yan P . Lu J . Song G . Zhu Y . Li Z . Zhao Y . Shen B . Huang X . Zhu H . Orkin SH . Shen X .
Tsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, China; School of Life Sciences, Peking University, Beijing 100871, China.
来源: Cell Stem Cell ( P 1934-5909 E 1875-9777 ) IF:22.387(2015) H指数:85 年: 2015 卷: 16 期: 5 页: 504-16
PMID: 25891907 [Pubmed] 细胞生物学,1区

B cell super-enhancers and regulatory clusters recruit AID tumorigenic activity. 百度翻译

Qian J . Wang Q . Dose M . Pruett N . Kieffer-Kwon KR . Resch W . Liang G . Tang Z . Mathé E . Benner C . Dubois W . Nelson S . Vian L . Oliveira TY . Jankovic M . Hakim O . Gazumyan A . Pavri R . Awasthi P . Song B . Liu G . Chen L . Zhu S . Feigenbaum L . Staudt L . Murre C . Ruan Y . Robbiani DF . Pan-Hammarström Q . Nussenzweig MC . Casellas R .
Genomics and Immunity, NIAMS, NIH, Bethesda, MD 20892, USA.
来源: Cell ( P 0092-8674 E 1097-4172 ) IF:32.242(2014) H指数:503 年: 2014 卷: 159 期: 7 页: 1524-37
PMID: 25483777 [Pubmed] 生化与分子生物学,1区

Targeted gene correction minimally impacts whole-genome mutational load in human-disease-specific induced pluripotent stem cell clones. 百度翻译

Suzuki K . Yu C . Qu J . Li M . Yao X . Yuan T . Goebl A . Tang S . Ren R . Aizawa E . Zhang F . Xu X . Soligalla RD . Chen F . Kim J . Kim NY . Liao HK . Benner C . Esteban CR . Jin Y . Liu GH . Li Y . Izpisua Belmonte JC .
Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
来源: Cell Stem Cell ( P 1934-5909 E 1875-9777 ) IF:22.268(2014) H指数:85 年: 2014 卷: 15 期: 1 页: 31-6
PMID: 24996168 [Pubmed] 细胞生物学,1区

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. 百度翻译

Zhou Y . Zhu S . Cai C . Yuan P . Li C . Huang Y . Wei W .
1] State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China [2].
来源: Nature ( P 0028-0836 E 1476-4687 ) IF:41.456(2014) H指数:734 年: 2014 卷: 509 期: 7501 页: 487-91
PMID: 24717434 [Pubmed] 综合性期刊,1区

10.

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.百度翻译

Shen B . Zhang W . Zhang J . Zhou J . Wang J . Chen L . Wang L . Hodgkins A . Iyer V . Huang X . Skarnes WC .
1] Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China. [2].
来源: Nat Methods ( P 1548-7091 E 1548-7105 ) IF:32.072(2014) H指数:112 年: 2014 卷: 11 期: 4 页: 399-402
PMID: 24584192 [Pubmed] 生化研究方法,1区

11.

TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys. 百度翻译

Liu H . Chen Y . Niu Y . Zhang K . Kang Y . Ge W . Liu X . Zhao E . Wang C . Lin S . Jing B . Si C . Lin Q . Chen X . Lin H . Pu X . Wang Y . Qin B . Wang F . Wang H . Si W . Zhou J . Tan T . Li T . Ji S . Xue Z . Luo Y . Cheng L . Zhou Q . Li S . Sun YE . Ji W .
Yunnan Key Laboratory of Primate Biomedical Research, Kunming, 650500, China; Translational Stem Cell Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200092, China.
来源: Cell Stem Cell ( P 1934-5909 E 1875-9777 ) IF:22.268(2014) H指数:85 年: 2014 卷: 14 期: 3 页: 323-8
PMID: 24529597 [Pubmed] 细胞生物学,1区

12.

Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. 百度翻译

Niu Y . Shen B . Cui Y . Chen Y . Wang J . Wang L . Kang Y . Zhao X . Si W . Li W . Xiang AP . Zhou J . Guo X . Bi Y . Si C . Hu B . Dong G . Wang H . Zhou Z . Li T . Tan T . Pu X . Wang F . Ji S . Zhou Q . Huang X . Ji W . Sha J .
Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650500, China; Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China.
来源: Cell ( P 0092-8674 E 1097-4172 ) IF:32.242(2014) H指数:503 年: 2014 卷: 156 期: 4 页: 836-43
PMID: 24486104 [Pubmed] 生化与分子生物学,1区

13.

Conditional targeted genome editing using somatically expressed TALENs in C. elegans.百度翻译

Cheng Z . Yi P . Wang X . Chai Y . Feng G . Yang Y . Liang X . Zhu Z . Li W . Ou G .
1] National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China. [2] School of Life Sciences, Peking University, Beijing, China. [3].
来源: Nat Biotechnol ( P 1087-0156 E 1546-1696 ) IF:39.08(2013) H指数:251 年: 2013 卷: 31 期: 10 页: 934-7
PMID: 23955274 [Pubmed] 生物工程与应用微生物,1区

14.

Comprehensive analysis of RNA-Seq data reveals extensive RNA editing in a human transcriptome. 百度翻译

Peng Z . Cheng Y . Tan BC . Kang L . Tian Z . Zhu Y . Zhang W . Liang Y . Hu X . Tan X . Guo J . Dong Z .
BGI-Shenzhen, Shenzhen, China.
来源: Nat Biotechnol ( P 1087-0156 E 1546-1696 ) IF:32.438(2012) H指数:251 年: 2012 卷: 30 期: 3 页: 253-60
PMID: 22327324 [Pubmed] 生物工程与应用微生物,1区