https://www.sciencedirect.com/science/article/abs/pii/S1360138517300882

摘要

Recent advances in next-generation sequencing technology have boosted the field of plant–microorganism interactions, especially in rhizosphere ecology.

高通量测序技术近期的发展促进了植物-微生物互作、特别是根际生态学这一领域的繁荣。

The principle of a core microbiota has been proposed to describe the microbial community that is systematically associated with a given plant genotype.

现在我们原则上认为每种基因型的植物都有自己特有的核心微生物群。

So far, this core microbiota was mostly defined on the basis of DNA sequences with taxonomic value, and not on their functional relevance.

这样的核心微生物群目前主要还是建立在以分类阶元为标记的系统之上，而很少探讨他们的功能性。

However, biogeography studies suggest that microbial reservoirs may differ depending upon soil types, thus questioning the universal distribution of the taxonomic core microbiota under various environmental conditions.

然而生物地理学研究表明土壤类型导致不同地区土壤微生物组成不一致，从而让不同生境中是否真的存在分类意义上广布的核心微生物群存疑。

The microbiota recruited by a given plant genotype in different environments seems to share greater functional similarity than taxonomic similarity.

事实上，同一基因型的植物在不同生境下所募集到的微生物群具有更多功能上而不是分类上的相似性。

所以，扩增子测序不适合大尺度的拉通对比，宏基因组测序势在必行，只是更有效的总DNA提取还有待方法上的简化和改进呀。