过去该小组曾经发表呼吸氢气治疗大鼠颅脑创伤，刚刚发表的论文改变给氢气的途径，通过注射氢气生理盐水，采用类似的研究方案，证明氢气对脑创伤具有治疗作用。主要观察指标包括血脑屏障、神经功能和抗氧化酶。这样的研究本身没有问题，但只是改变给药方法，观察同样的指标，几乎证明同样一个问题。

这样的论文，无论是做，还是写都相对容易，但从科学性上不应该鼓励。至少应该对比两种不同的给药方法是否存在不同，否则给人一种克隆论文的感觉，这样会影响在国际同行中的声誉。

在我们的学术水平和国外差距比较大的情况下，我们有不少研究是属于追踪和模仿，难免在科学性和创新性上存在差距，也是许多研究论文无法在高等次杂志上发表的重要原因。经过一段时间的追踪和模仿，我们应该尽量和国际上同步，甚至要在学术思想和科研设计上提出新的思路和模式，以建立我们在国际上的学术地位和影响力。最要不得的是我们自己完全重复自己，做灌水论文，这样对学术进步不仅效果不好，而且可能会影响学术发展。应该尽量避免这类工作。

Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury Via reducing oxidative stress

Abstract

Background

Hydrogen gas (H2) has been considered as a novel antioxidant to selectively reduce the toxic reactive oxygen species (ROS) such as hydroxyl radical (•OH) without affecting the other signal ROS. Our recent study shows that H2 inhalation is beneficial to traumatic brain injury (TBI) via reducing oxidative stress. In contrast to H2, hydrogen-rich saline (HS) may be more suitable for clinical application. The present study was designed to investigate whether HS has a protective effect against TBI via reducing oxidative stress in rats.

Methods

TBI model was induced by controlled cortical impact injury. Different dosages of HS were intraperitoneally administered at 5 min after TBI operation. We then measured the brain edema, blood-brain barrier (BBB) breakdown, neurological dysfunction and injury volume in all animals. In addition, the oxidative products and antioxidant enzymes in brain tissues were detected.

Results

TBI-challenged rats exhibited significant brain injuries characterized by the increase of BBB permeability, brain edema, and lesion volume as well as neurological dysfunction, which were dose-dependently ameliorated by HS treatment. Moreover, we found that HS treatment increased the endogenous antioxidant enzymatic activities and decreased the oxidative product levels in brain tissues of TBI-challenged rats.

Conclusion

Hydrogen-rich saline can exert a protective effect against TBI via reducing oxidative stress. Molecular hydrogen may be a more effective therapeutic strategy for TBI patients.