氢气治疗治疗噪声性耳聋的论文目前已经有三篇，前2篇是来自日本的研究，这是来自中国的研究，从研究的深度上，这个是相对细致的，分别从宏观的功能学脑干诱发电位，和微观的DPOAE听毛细胞功能，并进行形态学观察检测细胞损失的情况。虽然研究的相对好一些，当仍没有摆脱氢气研究的描述性性质。

文章是来自长海医院耳鼻外科周义德教授，文章被Neuroscience接受并在线发表。他于今年刚获得一项氢气治疗耳聋方面的国家自然科学基金。另外，国内有来自301医院耳鼻科研究所的中文论文也是关于氢气治疗噪声性耳聋的研究。西京医院也有关于氢气分子对噪声性耳聋氧化损伤的防治http://www.fuyin120.cn/newInfo.aspx?new_id=11678

Hydrogen-rich saline alleviates experimental noise-induced hearing loss in guinea pigs

Yide Zhoua, Hongliang Zhenga, Fangming Ruanb, Xiao Chenc, Guiliang Zhenga, Manman Kangb, Qing Zhanga, Xuejun Sunc,

a Department of Otolaryngology Head, Neck surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China

Abstract

Objective

To examine the efficiency of hydrogen-rich saline in the treatment of intensive noise-induced cochlear injury.

Materials and methods

Forty guinea pigs were assigned to one of four groups: HS+NOISE (intraperitoneal [i.p.] injection hydrogen-rich saline), NS+NOISE (i.p. injection normal saline), NOISE ALONE (noise control), and NO TREATMENT (normal control) groups. The HS+NOISE, NS+NOISE, and NOISE ALONE groups were exposed to intensive noise (4 h at 115 dB SPL noise of 4000±100 Hz). The auditory brainstem response (ABR) was used to examine the hearing threshold in each group. Distortion product otoacoustic emission (DPOAE) was used to examine outer hair cell function. We also examined cochlear morphology to evaluate inner and outer hair cell trauma induced by noise exposure. Hydrogen-rich saline was administered twice daily for 6 days (2.5 ml/kg i.p.) 24 h after noise exposure.

Results

Baseline ABR thresholds and DPOAE values were normal in all groups at the measured frequencies (2, 4, 8, and 16 kHz) before noise exposure. The ABR threshold shift was 50–55 dB across the frequencies tested, and average DPOAE declined in the NOISE ALONE, NS+NOISE, and HS+NOISE groups 24 h after noise exposure. However, the changes in cochlear parameters were different between groups. The HS+NOISE group showed a significantly decreased ABR threshold value as compared with the NS+NOISE or NOISE ALONE group ( P <0.01) on day 7. The mean DPOAE recovered to some extent in the three noise exposure groups, but at most frequencies the HS+NOISE group showed significantly increased DPOAE on day 7 as compared with the NS+NOISE group or NOISE ALONE group ( P <0.01). Surface Corti organ preparations stained with succinate dehydrogenase (SDH) showed that most outer hair cells (OHCs) were still dropsical and a few were missing 7 days after noise exposure in the NS+NOISE group. Only a few OHCs were slightly dropsical in the HS+NOISE group. The numbers of missing hair cells 7 days after noise exposure were significantly greater in the NOISE ONLY and NS+NOISE groups than the HS+NOISE group ( P <0.01).

Conclusion

Hydrogen-rich saline can alleviate experimental noise-induced hearing loss in guinea pigs, partially by preventing the death of cochlear hair cells after intensive noise exposure.