MAG Media Reagents (per liter)

1.1g MES [2-(N-Morpholino)- ethanesulfonic acid，吗啉代乙磺酸, SIGMA货号M3671/M2933,产品CAS号:4432-31-9，生物缓冲剂] 

1.3g HEPES [4-(2-hydroxyethyl)-1-Piperazineethanesulfonic acid, Hepes, 羟乙基哌嗪乙硫磺酸,一种缓冲液]

1.0g Arabinose (DL)

1.0g Gluconic Acid~Sodium Salt)

1.0g Yeast extract

2ml KH₂PO₄ Solution (110g/L)

4ml Na₂SO₄ Solution (62.5 g/L)

1ml MgSO₄ • 7H₂O Solution (180g/L)

2ml NH₄Cl Solution (160g/L)   

      1ml CaCl₂ Solution (13g/L)

1ml FeCl₃ • 6H₂O Solution (6.7g/L)

Adjust pH to 6.6 with KOH

Autoclave for 40 minutes

MAG培养基来自文献：

Sachs, J.L., Kembel, S.W., Lau, A.H., Simms, E.L. (2009) In Situ phylogenetic structure and diversity of wild Bradyrhizobium communities. Appl. Environ. Microbiol. 75, 4727-4735.

在Sachs的新的一篇论文中提到，使用MAG培养基可以提高日本慢生根瘤菌(Bradyrhizobium japonicum)的生长速率(MAG media was chosen because B. japonicum grows rapidly in the medium.)。该文献如下：

Sachs, J.L., Russell, J.E., Hollowell, A.C. (2011) Evolutionary instability of symbiotic function in Bradyrhizobium japonicum. PLoS One 6, e26370. 【这篇文献也是慢生根瘤菌试验进化的好文献，值得参考！】