来自山东泰山医学院动脉粥样硬化研究所的研究，最近在线发表在Metabolism和  Atherosclerosis杂志上，该研究所在泰山学者秦树存教授带领下，先后在肺动脉高压、动脉硬化、视网膜兴奋毒性等方面证明了氢气的保护效应。目前已经发表国际论文5篇，从研究技术和研究的深度上，他们的系列研究都达到国际上该领域的同样水平，并在许多方面明显领先于其他实验室。这些研究把中国氢气生物学研究推进了一个档次。从发表论文的数量看，是中国各研究单位中继第二军医大学和第四军医大学（5篇）以外发表论文数量最多的单位。关于氢气对动脉硬化作用的研究，08年日本学者太田教授曾经发表过对apo E基因敲除模型的效应观察，发表在BBRC上，后来他们发表关于肥胖的研究与此相关。其他单位尚没有关于氢气治疗动脉硬化方面的研究。

    发表在Atherosclerosis的论文也采用了apo E基因敲除模型，但在机制研究上远远超过日本学者的研究。该系列研究将氢气在动脉硬化预防和治疗的基础研究大大推进了一步，标志着我国学者在氢气与动脉硬化研究方面领先于国际水平。

    本研究在过去关于氢气具有抗动脉硬化研究的基础上，针对脂蛋白的含量和成分进行了深入研究，首次证明了氢气可以促进泡沫细胞释放脂蛋白，同时能减少脂蛋白的氧化水平。这不仅对解释氢气治疗动脉硬化的机制，而且对研究氢气的生物学效应的机制都具有重要意义。

Metabolism
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Administration of hydrogen-saturated saline decreases plasma low-density lipoprotein cholesterol levels and improves high-density lipoprotein function in high-fat diet–fed hamsters

Chuanlong Zong^1, a, Guohua Song^1, a, Shutong Yao^a, Luqin Li^a, Yang Yu^a, Lei Feng^a, Shoudong Guo^a, Tian Luo^a, Shucun Qin ^, a, 

Received 27 July 2011; Accepted 19 October 2011. Available online 6 December 2011.

Abstract

Hydrogen (dihydrogen; H₂) has an antiatherosclerotic effect in apolipoprotein (apo) E knockout mice. The goals of this study were to further characterize the effects of H₂ on the content, composition, and biological activities of plasma lipoproteins in golden hamsters. Plasma analysis by enzymatic method and fast protein liquid chromatography showed that 4-week intraperitoneal injection of hydrogen-saturated saline remarkably decreased plasma total cholesterol and low-density lipoprotein (LDL) cholesterol levels in high-fat diet–fed hamsters. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of apolipoproteins from ultracentrifugally isolated plasma lipoproteins revealed a marked decrease of apo B100 and apo B48 in LDL. A profound decrease of apo E level in very low-density lipoprotein was also observed. Besides, we determined the functional quality of high-density lipoprotein (HDL) particles isolated from H₂-treated and control mice. H₂ significantly improved HDL functionality assessed in 2 independent ways, namely, (1) stimulation of cholesterol efflux from macrophage foam cells by measuring HDL-induced [³H]cholesterol efflux and (2) protection against LDL oxidation as a measure of Cu²⁺-induced thiobarbituric acid reactive substances formation. Administration of hydrogen-saturated saline decreases plasma LDL cholesterol and apo B levels and improves hyperlipidemia-injured HDL functions, including the capacity of enhancing cellular cholesterol efflux and playing antioxidative properties, in high-fat diet–fed hamsters.

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·         1. Introduction

·         2. Methods

·          

o    2.1. Preparation of hydrogen-saturated saline

o    2.2. Animals and experimental design

o    2.3. Plasma lipid analysis

o    2.4. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of apolipoproteins

o    2.5. HDL-induced cholesterol efflux assay

o    2.6. HDL antioxidation capability measurement

o    2.7. Statistical analysis

·         3. Results

·          

o    3.1. Administration of H₂ lowers plasma TC and LDL-C levels

o    3.2. H₂ downregulates apo B and apo E protein levels in plasma

o    3.3. Administration of H₂ improves the functionality of HDL particle in hyperlipidemic hamsters

·         4. Discussion

·         Funding

·         Conflict of Interest

·         References