T-DNA插入突变，是一种常用的大规模的产生突变体的方法。其基本原理是：T-DNA插入到功能基因，产生功能失活突变。但T-DNA的插入可能还会有其它效应，如这篇文章报道的：Chromosomal Translocations are a Common Phenomenon in Arabidopsis thaliana T-DNA Insertion Lines。Chromosomal Translocations 估计用不了多久还会有篇文章出来Chromosomal Translocations are a Common Phenomenon in rice T-DNA Insertion Lines。这种事情如果只发生在拟南芥好象有点说不通，水稻中应该有类似的情况。

      从T-DNA插入系再扩展到转基因，转基因植物中可能也会有类似情况

资料：Translocations

In genetics, a chromosome translocation is a chromosome abnormality caused by rearrangement of parts between nonhomologous chromosomes. A gene fusion may be created when the translocation joins two otherwise separated genes, the occurrence of which is common in cancer. It is detected on cytogenetics or a karyotype of affected cells. There are two main types, reciprocal (also known as non-Robertsonian) and Robertsonian. Also, translocations can be balanced (in an even exchange of material with no genetic information extra or missing, and ideally full functionality) or unbalanced (where the exchange of chromosome material is unequal resulting in extra or missing genes).

http://en.wikipedia.org/wiki/Chromosomal_translocation

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     除此外还有其它效应

Unexpected silencing effects from T-DNA tags in Arabidopsis

silencing effects

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