这个工作具有非常好的原始创新性。值得阅读，值得关注，值得推荐。

   日本医科大学太田小组目前也在开展老年性痴呆氢气水治疗的研究，他们在刚举行的学术会议中已经介绍了该工作。特别值得注意的是，日本学者已经开展了一些临床的研究，他们在会议中介绍了氢气对老年性认知障碍的研究，给我留下非常深刻的印象。这个研究是来自中国医科大学附属医院神经内科曹云鹏课题组，采用脑内注射amyloid β的痴呆模型，腹腔注射氢气盐水，采用炎症因子测定，行为学评价，氧化损伤指标和LTP电生理指标，确定了治疗效果，这个研究的指标和新意都比较突出，评我的估计，这个内容发表在BR上比较委屈。将来这个文章将会有比较好的引用情况。因为这个领域将是比较重要的研究方向。一是这个疾病比较重要，另一个是抗炎症是这个疾病将来比较有前途的治疗手段。如果氢气在这个疾病能得到临床证据，将是比较有前景的。

 

Hydrogen-rich Saline Improves Memory Function in a Rat Model of Amyloid-beta-induced Alzheimer’s Disease by Reduction of Oxidative Stress

 

Jian LIa, Cai WANGa, John H. Zhangb, Jian-mei CAIc, Yun-peng CAOa, Xue-jun SUNc

 

aDepartment of Neurology, the first affiliated hospital of China Medical University, Shengyang 110001, China; bDepartment of Neurosurgery, Loma Linda University, California, CA, USA; cDepartment of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, PR China

 

Corresponding authors: Yun-peng CAO, Department of neurology, First afflicted Hospital, China Medical University, Shenyang, 110001, PR China.

Telephone: (86)2483282516, Fax: (86)2483282385. E-mail: ypengcao@yahoo.com;

Xue-jun Sun, Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, PR China. Tel: (86)2125070349. Fax: (86)2165492382. Email: sunxjk@hotmail.com

 

Abstract

This study is to examine if hydrogen-rich saline reduced amyloid β (Aβ) induced neural inflammation, and learning and memory deficits in a rat model. S-D male rats (n = 84, 280-330g) were divided into three groups, sham operated, Aβ1-42 injected and Aβ1-42 plus hydrogen-rich saline treated animals. Hydrogen-rich saline (5 ml/kg, i.p., daily) was injected for 14 days after intracerebroventricular injection of Aβ1-42. The levels of MDA, IL-6 and TNF-α were assessed by biochemical and ELISA analysis. Morris Water Maze and open field task were used to assess the memory dysfunction and motor dysfunction, respectively. LTP were used to detect the electrophysiology changes, HNE and GFAP immunohistochemistry were used to assess the oxidative stress and glial cell activation. After Aβ1-42 injection, the levels of MDA, IL-6, and TNF-α were increased in brain tissues and hydrogen-rich saline treatment suppressed MDA, IL-6, and TNF-α concentration. Hydrogen-rich saline treatment improved Morris Water Maze and enhanced LTP in hippocampus blocked by Aβ1-42. Furthermore, hydrogen-rich saline treatment also decreased the immunoreactivitiy of HNE and GFAP in hippocampus induced by Aβ1-42. In conclusion, hydrogen-rich saline prevented Aβ-induced neuroinflammation and oxidative stress, which may contribute to the improvement of memory dysfunction in this rat model.

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