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#编者信息
熊荣川
明湖实验室
xiongrongchuan@126.com
http://blog.sciencenet.cn/u/Bearjazz
Accurate identification of plant species is a key component of biological, ecological, and evolutionary studies. Standard morphological techniques can be ineffective, especially for large and complex taxa (Liu et al., 2017; Percy et al., 2014; Yan et al., 2015), particular life stages (Gonzalez et al., 2009), or when only plant fragments are available (Jones et al., 2011; Kesanakurti et al., 2011). To support morphological identification, DNA barcoding has become widely used for the identification and confirmation of plant species (Fazekas et al., 2008; Hollingsworth et al., 2009; Kress & Erickson, 2007; Kress, Wurdack, Zimmer, Weigt & Janzen, 2005; Li et al., 2011). The plastid DNA regions rbcL, matK, trnH-psbA and the internal transcribed spacers (ITS) of nuclear ribosomal DNA have previously been used in systematic studies at various taxonomic levels (Azani et al., 2017; Baldwin et al., 1995; Chase et al., 1993; Harrington, Edwards, Johnson, Chase & Gadek, 2005), as well as for the taxonomy and species identification of specific target families and genera (Liu, Moeller, Gao, Zhang & Li, 2011; Xu et al., 2015; Yan et al., 2015). Increasingly, surveys of local (Kress et al., 2009) and regional (Burgess et al., 2011; De Vere et al., 2012; Liu, Yan et al., 2015) floras are based on DNA barcoding with these regions.
| 植物物种的准确鉴定是生物学、生态学和进化研究的关键组成部分。经典的形态学方法对于大型和复杂的分类群可能无效(Liu et al., 2017; Percy et al., 2014; Yan et al., 2015),尤其是在特定的生命阶段(Gonzalez et al., 2009),或者只有植物碎片可用时(Jones et al., 2011; Kesanakurti et al., 2011)。为了补充支持形态鉴定,DNA条形码已广泛用于植物物种的鉴定和确认(F Fazekas et al., 2008; Hollingsworth et al., 2009; Kress & Erickson, 2007; Kress, Wurdack, Zimmer, Weigt & Janzen, 2005; Li et al., 2011)。质体DNA区域rbcL, matK, trnH-psbA和细胞核核糖体DNA的内转录间隔区(ITS)之前被用于不同分类水平的系统学研究(Azani et al., 2017; Baldwin et al., 1995; Chase et al., 1993; Harrington, Edwards, Johnson, Chase & Gadek, 2005),特定目标科属的分类学研究以及和物种鉴定研究(Liu, Moeller, Gao, Zhang & Li, 2011; Xu et al., 2015; Yan et al., 2015)。越来越多的地方(Kress et al., 2009)和区域(Burgess et al., 2011; De Vere et al., 2012; Liu, Yan et al., 2015)植物区系的调查使用这些片段作为DNA条形码。 |
Shao-Lin T , Ya-Huang L , Hollingsworth P M , et al. DNA barcoding herbaceous and woody plant species at a subalpine forest dynamics plot in Southwest China[J]. Ecology and Evolution, 2018. |
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