ECV304是在生命科学尤其是基础医学领域非常常用的一种细胞，它一直被研究者误认为是血管内皮细胞，直到现在很多人甚至教授、博导都仍然把它当作血管内皮细胞用于研究。虽然我早就听说ECV304不是真正的血管内皮细胞，但是一直没有找到相关的资料。今天在丁香园看到关于“ECV304不是血管内皮细胞”的权威资料，仔细阅读了才知道原来ECV304其实是膀胱癌细胞派生的细胞。在此转贴丁香园的原帖内容，谢谢发帖者SDhchina！

ECV304 不是 血管内皮细胞 的 权威资料
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cox2wj 说的对，ECV其实是膀胱癌细胞派生的细胞，我上ATCC网页问的ATCC公司，当时也是因为奇怪他们怎么不卖ECV304了，以下是ATCC的EMAIL回信原文：
Thank you for contacting the ATCC.
Unfortunately, CRL-1998 is no longer available for distribution.
ECV-304 was deposited as a human endothelial cell line, however, recent
DNA profiling and cytogenetic studies show that it is a derivative of the
T24 bladder carcinoma (ATCC HTB-4). ( derived from the same individual)
I hope that this information is helpful to you.
Please feel free to contact us if you have any other questions.
tiger刘
zhaoqingshan edited on 2004-11-09 20:50
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Recently, my classmates E-mail me about ECV-304 Cell.
The cell in the cell line ECV-304 are not endothelial cell, but are derivated from the tumor cells of a patient with bladder carcinoma.
Some papers about it:
1．Dirks WG, Macleod RAF, Drexler HG. ECV-304(ENDOTHELIAL)is really T24(bladder carcinoma):cell line cross-contamination at source. In Vitro Cell Dev Biol 35:558~559 1999;
2. Drexler HG, Quentmerier H,Driks WG et al. Bladder carcinoma cell line ECV-304 is not a model system for endothelial cells.In Vitro Cell Dev Biol 38:185-186,2002;
3. Brown J, Reading SJ, Jones S et al. Ciritical evaluation of ECV-304 as a human and functional responses:A comparison with the human bladder cancer derived epithelial line T24/83. Lab Invest 2000;80:37-45
4. Tanabe H, Takada Y, Minegishi D, et al. All line individualization by SIR multiplex system in the cell bank found cross-contamination between ECV-304 and EJ-T24. Tiss cult Res Commun 1999;18:329-338;
If you want to get more informatin about it ,please visit the following website:
http://www.dsmz.de/mutz/mutz310.htm
http://www.ecacc.org.uk
using key word: ECV ,then you will get some informations.
Lewis_1121 edited on 2003-09-09 14:10
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JCRB0744 [ECV304]
Profile:Originally this cell line was established as human endothelium cells from umbilical cord but later the cell line was identified to be an EJ1/T24 by STR-PCR analysis.

Date accepted: 04/26/1991
Animal: human
Sex and Age: M :0-years-old
Scientific Name: Homo sapiens
Case history: normal
Classification: transformed
Tissue prepared: umbilical cord
History of the cell: Cells were prepared from umbilical cord epithelium by the treatment of Dispase.
Medium: M199 medium with 10% fetal bovine serum.
Passage method: Cells are hrvested after treatment with 0.125% trypsin and 0.02% EDTA.
CO2 Concentration: 5 %
Cell no. at passage: 7.3 x 10^3 cells/sq.cm.
Genetics: human immortalized endothelium cell
Life span: infinite
Morphology: epithelial-like
Characteristics: human immortalized endothelium cell line from umbilical cord. But JCRB identified the cell line is derived from cross contamination with famous EJ1/T24 cells by STR-PCR analysis.
Cell ID data: available
DNA Profile (STR): D5S818:10
D13S317:12
D7S820:10,11
D16S539:9
vWA:17
TH01:6
Amelogenin
TPOX:8,11
CSF1PO:12

Virus DNA detected: CMV(-), EBV(-), HHV6(-), HHV7(-), BKJ(-), JCV(-), ADV1(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] -- Description
Established by Takahashi,K.
Deposited by Umeda,M.
Special notice: Refer Ref.(1) when publish paper using the cell line ECV304.
Cell bank: YCBR(JCR
References: (1,2,3,4)
Comments: Dr. Drexler of the DSM informed to us that the ECV304 was crosscontaminated with EJ1/T24 at the end of 1998. Following his suggestion the JCRB examined by STR-PCR method and confirmed the crosscontamination.