发表生物微流体Biomicrofluidics上一篇有关直接捕获全血中白细胞的微流控芯片的论文

Amicrofluidic chip for direct and rapid trapping of white blood cells from wholeblood

Jingdong Chen, Di Chen, Tao Yuan, Yao Xie and Xiang Chen.

A microfluidic chip for direct and rapid trapping of white blood cells from whole blood.pdf

摘要：

   设计和制备了基于化学分离及微结构阵列集成的白细胞捕获芯片。此芯片包括两个功能单元：红细胞裂解单元和白细胞捕获单元。芯片的红细胞裂解单元是一条由52个半圆环连接而成的弯曲管道。全血中的红细胞经过与红细胞裂解液在弯曲管道中充分混合后而裂解。两者在约为0.05 μl/min的流速下能够在弯曲管道达到完全混合，混合时间约为85 s。较大体积的红细胞、较高浓度的全血和较长的红细胞裂解液与红细胞之间的反应时间导致了全血和红细胞裂解液混合并使红细胞裂解的时间更长，因此全血和红细胞完全混合时流速比柠檬黄和明亮蓝的水溶液完全混合时的流速小。红细胞完全裂解后的血液流入白细胞捕获单元，其中的白细胞被捕获结构阵列捕获。利用此芯片达到了从全血中直接捕获白细胞的目的，而且有利于后续功能的集成。

Abstract:

Blood analysis plays a major role in medical and science applications and white blood cells (WBCs) are most often the target of analysis. We proposed an integrated microfluidic chip for direct and rapid trapping WBCs from whole blood. The microfluidic chip consists of two basic functional units: a winding channel to mix and arrays of two-layer trapping structures to trap WBCs. Red blood cells (RBCs) were eliminated through moving the winding channel and then WBCs were trapped by the arrays of trapping structures. We fabricated the PDMS (polydimethylsiloxane) chip using soft lithography and determined the critical flow velocities of tartrazine and brilliant blue water mixing and whole blood and red blood cell lysis buffer mixing in the winding channel. They are 0.25 μl/min and 0.05 μl/min, respectively. The critical flow velocity of the whole blood and red blood cell lysis buffer is lower due to larger volume of the RBCs and higher kinematic viscosity of the whole blood. The time taken for complete lysis of whole blood was about 85 s under the flow velocity 0.05 μl/min. The RBCs were lysed completely by mixing and the WBCs were trapped by the trapping structures. The chip trapped about 2.0×10³ from 3.3×10³ WBCs.

http://scitation.aip.org/content/aip/journal/bmf/7/3/10.1063/1.4808179