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建立DH群体的新方法

已有 6783 次阅读 2012-3-14 11:49 |个人分类:技术和方法|系统分类:科研笔记

其实也不是太新了.最早的报道应该是这篇文章:
Ravi, M., and Chan, S.W.L. Haploid plants produced by centromere-mediated genome elimination. Nature 464, 615-618.

Haploid plants produced by centromere-mediated.pdf
这东西,我看见我就比较有兴趣.拟南芥里可以,水稻里应该是同样的道理.

现在拟南芥已经用这种方法来建立DH群体了
Seymour, D.K., Filiault, D.L., Henry, I.M., Monson-Miller, J., Ravi, M., Pang, A., Comai, L., Chan, S.W.L., and Maloof, J.N. Rapid creation of Arabidopsis doubled haploid lines for quantitative trait locus mapping. Proceedings of the National Academy of Sciences 109, 4227-4232.

Rapid creation of Arabidopsis doubled haploid lines.pdf

相关的文献还有这两篇
Haploidy with histones.

Haploidy with histones.pdf
Ravi, M., Shibata, F., Ramahi, J.S., Nagaki, K., Chen, C., Murata, M., and Chan, S.W.L. Meiosis-Specific Loading of the Centromere-Specific Histone CENH3 in <italic>Arabidopsis thaliana</italic>. PLoS Genet 7, e1002121.

Meiosis-Specific Loading of the Centromere-Specific.pdf

呵呵,其它作物该行动了.



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