Large-scale detection of rare variants via pooled multiplexed next-generation sequencing: towards next-generation Ecotilling

Large-scale detection of rare variants towards next-generation Ecotilling.pdf

EcoTILLING的本质:在大自然群体中高通量地发现特定基因(已知序列,但表型不一定知道)的等位基因(基于序列的等位基因)的方法.所以发现等位基因应该是为下一步的深入研究奠定基础.