A visual peroxidase mimicking aptasensor based on Pt nanoparticles-loaded on iron metal organic gel for fumonisin B<sub>1</sub> analysis in corn meal.
By:
Li, Mei; Li, De Yan; Li, Zi Ying; Hu, Rong; Yang, Yun Hui; Yang, TongBiosensors & bioelectronics
Volume 209
Page 114241
DOI 10.1016/j.bios.2022.114241
Published 2022-Apr-05
Indexed 2022-04-13
Document Type
Journal Article
Abstract
A visual colorimetric aptasensor combining platinum nanoparticles (Pt NPs) loaded on Fe-based metal organic gel (Fe-MOG) as peroxidase mimics with magnetic separation technique was designed for the detection of fumonisin B1 (FB1). Interestingly, based on the inherent properties of Fe-MOG such as porous nanostructures, abundant Fe2+/Fe3+ active metal centers and synergetic effect between Pt NPs and Fe-MOG, Pt NPs/Fe-MOG composites can be employed as excellent peroxidase mimetic enzyme to oxidize 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. This process was accompanied by a color variation of the solution from colorless to blue. Kinetic analysis showed that the Pt NPs/Fe-MOG composites make an effective peroxidase mimic with low Michaelis constant (Km), high substrate binding affinity and rapid catalytic velocity (Vm). Furthermore, the Pt NPs/Fe-MOG based colorimetric aptasensor was used to detect FB1. The prepared Pt NPs/Fe-MOG achieved lower detection limit (2.7pg/mL, 3sigma/k) with a wide linear range of 0.01-2000.0ng/mL. Finally, as a proof of concept of the rapidity and the simplicity of this colorimetric aptasensor, Pt NPs/Fe-MOG was used for the detection of FB1 in real corn samples.
Copyright © 2022 Elsevier B.V. All rights reserved.
Keywords
Keyword List
AptasensorColorimetric analysisCorn sampleFumonisin B(1) (FB(1))Metal-organic gels (MOGs)Peroxidase mimicking
Author Information
Addresses
College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China.College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China. Electronic address: yyhui2002@aliyun.com.College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China. Electronic address: yt09132149@163.com.
Journal information Biosensors & bioelectronics eISSN : 1873-4235
Li, Mei; Li, De Yan; (...); Yang, Tong2022-Apr-05 | Biosensors & bioelectronics 209 , pp.114241
A visual colorimetric aptasensor combining platinum nanoparticles (Pt NPs) loaded on Fe-based metal organic gel (Fe-MOG) as peroxidase mimics with magnetic separation technique was designed for the detection of fumonisin B1 (FB1). Interestingly, based on the inherent properties of Fe-MOG such as porous nanostructures, abundant Fe2+/Fe3+ active metal centers and synergetic effect between Pt NPs
Show moreView full text
Zhang, X; Chi, KN; (...); Yang, YHMar 15 2019 | BIOSENSORS & BIOELECTRONICS 129 , pp.64-71
In this study, a novel photoelectrochemical (PEC) aptasensor based on two-dimensional (2D) porphyrinic covalent organic frameworks (p-COFs) for the label-free detection of C-reactive protein (CRP) is presented. The obtained p-COFs possess high conductivity and an improved stability due to strong and rigid covalent linkages. The introduction of p-COFs hinder the recombination of electrons and ho
Show moreFull Text at Publisher49Citations49ReferencesRelated records
Hu, R; Zhang, X; (...); Zhang, XBJun 15 2017 | BIOSENSORS & BIOELECTRONICS 92 , pp.40-46
A universal aptameric system based on the taking advantage of double-stranded DNA/perylene diimide (dsDNA/PDI) as the signal probe was developed for multiplexed detection of small molecules. Aptamers are single-stranded DNA or RNA oligonucleotides which are selected in vitro by a process known as systematic evolution of ligands by exponential enrichment. In this work, we synthesized a new kind
Show moreView full text23Citations46ReferencesRelated records
转载本文请联系原作者获取授权，同时请注明本文来自蒋金和科学网博客。
链接地址：https://wap.sciencenet.cn/blog-454141-1334290.html?mobile=1
收藏
