氢气的抗氧化抗炎症损伤很容易让人联想到是否在器官移植方面的应用，目前国际上在氢气对抗器官移植损伤方面研究最多的美国匹大的Nakao小组，不过该教授目前在美国的实验已经停止，回到日本作临床医生，不了解他目前工作的情况如何。最近国内几家单位也相继做了一些氢气对器官移植损伤方面的研究，如上海长海医院关于心脏体外保护方面的研究，也有关于肾脏移植保护的研究，但总体上这个方面国内研究并不多。现在的文章来自哈尔滨医科大学附属医院的一研究，是让脑死亡动物呼吸2%的氢气氮气（48%）氧气（50%）混合气，对照组呼吸氮气（48%）氧气（50%）混合气。呼吸时间为2小时，受体动物也呼吸同样成分的气体。这个模式非常类似2008年美国关于小肠移植的研究，不过他们是呼吸3小时。采用组织学生物化学和蛋白分析等方法针对氧化损伤、组织损伤和炎症因子等进行检测，证明呼吸氢气确实能改善这样脑死亡动物肺抑制组织损伤的情况。

点评，属于跟踪性研究，其实作为研究，没有必要一定要强调脑死亡，一方面脑死亡对肺损伤的贡献到底有多少并不清楚，更重要的是本研究最需要了解的是器官移植本身造成的损伤是否受到保护，当然脑死亡更符合临床实际情况，但这会造成动物操作的复杂性，对明确氢气的效果并没有太大帮助。

Hydrogen inhalation decreases lung graft injury in brain-dead donor rats

BACKGROUND

The process of brain death induces acute lung injury in donors and aggravates ischemia–reperfusion injury (IRI) in grafts. Hydrogen, a new anti-oxidant, attenuates IRI in several organ transplant models. We examined whether 2% inhaled hydrogen would show favorable effects on lung grafts from brain-dead donor rats.

METHODS

Brain-dead donor rats inhaled mixed gases with either 50% oxygen and 50% nitrogen or mixed gases with 2% hydrogen, 50% oxygen and 48% nitrogen for 2 hours. The recipients inhaled the same gas as the donors and were euthanized 2 hours after lung transplantation.

RESULTS

上图一组为HE（左）一组为Tunel（右）染色。

Hydrogen improved PaO2/FIO2 and PVO2/FIO2 from the arterial and pulmonary venous blood in recipients and decreased the lung injury score in grafts from brain-dead donors. Hydrogen decreased the amount of IL-8 and TNF-α in serum, inhibited the activity of malondialdehyde and myeloperoxidase, and increased the activity of superoxide dismutase in the lung grafts from brain-dead donors. Furthermore, hydrogen decreased the apoptotic index of the cells and inhibited the protein expression of intercellular adhesion molecule-1 and caspase-3 in lung grafts from brain-dead donors.

CONCLUSIONS

Hydrogen can exert protective effects on lung grafts from brain-dead donors through anti-inflammatory, anti-oxidant and anti-apoptotic mechanisms.