Hydrogen saline is protective for acute lung.pdf

四川大学华西医院麻醉系刘进教授课题组2011年在《外科研究杂志》上发表了氢气治疗兔肺缺血再灌注损伤的文章。该实验室是国际上最早开展氢气研究的单位之一。最近上海胸科医院发表类似论文，证明注射氢气生理盐水可以治疗大鼠肺缺血再灌注损伤，该文章发表在Heart, Lung and Circulation杂志上，该研究证明注射氢气生理盐水（先连续提前3天，然后在缺血再灌注时注射0.5 ml/kg）可以治疗肺缺血再灌注组织损伤，作者分别采用肺组织干湿比（可以分析肺水肿程度）、肺动脉体外收缩和舒张功能（分别用乙酰胆碱和肾上腺素诱导血管的扩张和收缩功能）、HE染色（肺组织病理损伤程度）、TUNEL染色和caspase-3活性（显示肺组织细胞凋亡的程度）、MDA和8-OHdG（组织氧化损伤程度，分别代表脂质和核酸氧化损伤的程度）以及血液白细胞CRP等生物标志。等研究手段，证明氢气盐水注射对大鼠肺缺血再灌注损伤有治疗作用。

疑问：1、为什么要提前使用药物，什么原因？提前抗氧化有什么价值？如果有，为什么没有和单独损伤后进行对比，至少过去没有见过类似给药物的方法。2、氢气生理盐水剂量为什么采用0.5 ml/kg，有什么依据？3、我前面提到2011年就有四川华西医院发表氢气生理盐水对兔肺缺血再灌注损伤有治疗作用，本文为什么没有引用？本文投稿日期是2012年2月，应该可以看到该文献，从文献类似度上考虑，这是一篇绝对必须引用的文献。

Hydrogen saline is protective for acute lung ischaemia/reperfusion injuries in rats

Jianxin Shi1,

Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China

Protective effects of saturated hydrogen (H2) saline on cardiac ischaemia–reperfusion (I/R) injury have been demonstrated previously. This study was designed to show that hydrogen-rich saline is protective in preventing lung I/R injury in rats.

Adult male Sprague-Dawley rats underwent 45 min occlusion of the right lung roots and 120 min reperfusion. Rats were divided randomly into three groups: sham-operated control group, I/R plus saline treatment, and I/R plus hydrogen-rich saline treatment (0.6 mmol/L, 0.5 ml/kg/d). Three days of intraperitoneal injection of hydrogen-rich saline before the reperfusion combined with immediate administration of hydrogen-rich saline after the reperfusion were performed. Following reperfusion, the lung tissue and the pulmonary artery was immediately obtained and the W/D ratio, pulmonary artery contraction and relaxation ability, H–E staining, TUNEL staining, caspase-3, MDA, 8-OHdG content and measurement of such biomarkers as WBC, CRP were measured or carried out.

Results

Hydrogen saline significantly protected vasoactivity of the pulmonary artery, reduced pulmonary oedema, decreased lung malondialdehyde (MDA), 8-OHdG concentration, alleviated lung epithelial cell apoptosis and lowered the level of such biomarkers as WBC, CRP, ALT and TBiL.

Conclusions

It is concluded that hydrogen-rich saline is a novel, simple, safe and effective method to attenuate pulmonary I/R injury.

Hydrogen-Rich Saline Attenuates Lung Ischemia-Reperfusion Injury in Rabbits.pdf