郝兆东
The Plant Cell:非编码RNA HID1通过抑制ABA生物合成,促进phyB依赖性的种子萌发
2022-12-7 23:59
阅读:493

The noncoding RNA HIDDEN TREASURE 1 promotes phytochrome B-dependent seed germination by repressing abscisic acid biosynthesis

第一作者Yuqiu Wang

第一单位北京大学

通讯作者Danmeng Zhu


 Abstract 

背景回顾Light is a major environmental factor for seed germination. Red-light-activated phytochrome B (phyB) promotes seed germination by modulating the dynamic balance of two phytohormones, gibberellic acid (GA) and abscisic acid (ABA). 


提出问题How phyB modulates ABA biosynthesis after perceiving a light signal is not yet well understood. 


主要发现Here, we identified the noncoding RNA HIDDEN TREASURE 1 (HID1) as a repressor of ABA biosynthesis acting downstream of phyBduring Arabidopsis thaliana seed germination


结果1-HID1的功能Loss of HID1 function led to delayed phyB-dependent seed germination


结果2-HID1与phyB的关系Photo-activated phyBpromoted the accumulation of HID1 in the radicle within 48 hours of imbibition. Our transcriptomics analysis showed that HID1 and phyB co-regulate the transcription of a common set of genes involved in ABA and GA metabolism. 


结果3-HID1的抑制子Through a forward genetic screen, we identified three ABA biosynthesis genes, ABA DEFICIENT 1 (ABA1), ABA2, and ABA3, as suppressors of HID1


结果4-HID1-ATXR7-NCED9We further demonstrated that HID1 directly inhibits the transcription of 9-CIS-EPOXYCAROTENOID DIOXYGENASE (NCED9), a gene encoding a key rate-limiting enzyme of ABA biosynthesis. HID1 interacts with ARABIDOPSIS TRITHORAX-RELATED7 (ATXR7), an H3K4me3 methyltransferase, inhibiting its occupancy and H3K4me3 modification at the NCED9 locus. 


结论Our study reveals a nuclear mechanism of phyB signaling transmitted through HID1 to control the internal homeostasis of ABA and GA, which gradually optimizes the transcriptional network during seed germination.


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 摘 要 

光是影响种子萌发的主要环境因素。受红光激活的光敏色素phyB,通过调控赤霉素(GA)和脱落酸(ABA)之间的动态平衡,促进种子萌发。但是,phyB在感知光信号之后如何调控ABA的生物合成还不清楚。本文中,作者鉴定到了一个在拟南芥种子萌发过程中,作用于phyB下游的ABA生物合成抑制子,即非编码的RNA HID1HID1功能缺失突变会导致依赖于phyB的种子萌发延迟。光激活的phyB能够在种子吸胀的48小时内促进HID1在胚根中的表达。转录组分析显示,HID1和phyB共调控一些参与ABA和GA代谢基因的转录。通过一个正向遗传学筛选试验,作者鉴定到了3个ABA生物合成基因ABA1ABA2ABA3HID1的抑制子。作者进一步发现,HID1能够直接抑制ABA生物合成途径限速酶编码基因NCED9的转录。HID1与一个H3K4me3甲基转移酶ATXR7互作,抑制后者在NCED9位点上的占据,从而抑制其对NCED9的H3K4me3修饰。本文的研究结果揭示了一个核中的phyB信号转导机制,通过HID1控制内源GA和ABA的内稳态,从而在种子萌发过程中逐步优化转录网络。




** 丹萌 **

个人简介:

1998-2002年,北京大学,学士;

2002-2007年,北京大学,博士;

2004-2006年,耶律大学,交换生;

2007-2009年,北京生命科学研究所,研究助理;

2010年-至今,北京大学,副研究员。


研究方向

1. 非编码RNA参与高等植物生长发育及环境信号响应调控的分子机理;

2. 拟南芥光形态建成信号传导的分子机理。


doi: https://doi.org/10.1093/plcell/koac334


Journal: The Plant Cell

Published date: November 25, 2022


Cite:
Yuqiu Wang, Yangyang Fan, De Fan, Xiaoli Zhou, Yuntong Jiao, Xing Wang Deng, Danmeng Zhu, The noncoding RNA HIDDEN TREASURE 1 promotes phytochrome B-dependent seed germination by repressing abscisic acid biosynthesis, The Plant Cell, 2022;, koac334, https://doi.org/10.1093/plcell/koac334

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