On the trail of auxin: reporters and sensors

The phytohormone auxin is a master regulator of plant growth and development in response to many endogenous and environmental signals. The underlying coordination of growth is mediated by the formation of auxin maxima and concentration gradients. The visualization of auxin dynamics and distribution can therefore provide essential information to increase our understanding of the mechanisms by which auxin orchestrates these growth and developmental processes. Several auxin reporters have been developed to better perceive the auxin distribution and signaling machinery in vivo. This review focuses on different types of auxin reporters and biosensors used to monitor auxin distribution and its dynamics, as well as auxin signaling, at the cellular and tissue levels in different plant species. We provide a brief history of each reporter and biosensor group and explain their principles and utilities.

 摘 要 

植物激素生长素是植物响应多种内源和外部环境信号，调控自身生长和发育的主要调控子。植物生长的协调是通过形成生长素极大值点和浓度梯度来介导的。因此，生长素动态和分布的可视化可以为我们提供许多信息，用来加强对于生长素作用生长和发育进程机制的理解。为了更好地了解生长素在体内的分布和信号转导机制，研究者们已开发了几种生长素的报告子。本文综述了在不同植物中，细胞和组织水平上用于监测生长素分布、动态及信号转导的多种生长素报告子和生物感应器。作者详细介绍了每种生长素报告子和生物感应器的历史背景，并解释其工作原理和实践应用。