Molecular basis of (E)-β-farnesene-mediated aphid location in the predator Eupeodes corollae

第一作者：Bing Wang

第一单位：中国农科院植物保护研究所

第一通讯：Guirong Wang

Abstarct

背景回顾：(E)-β-farnesene (EBF) is an important chemical cue mediating interactions between plants, aphids, and natural enemies. This chemical has two origins, being secreted by aphid as an alarm pheromone and also produced by the attacked plants as a semiochemical attracting natural enemies.

(E)-β-金合欢烯（EBF）是一种重要的化学信号，介导植物、蚜虫及其天敌之间的相互关系。EBF具有两个来源，一个是由蚜虫分泌，作为一种报警信息素；另外一个来源则是由受到攻击的植物分泌，作为吸引蚜虫天敌的化学信息素。

提出问题：Despite the important role of this volatile chemical, little is known on the molecular mechanisms mediating the attraction of natural enemies to EBF.

尽管这种挥发性化学物质十分重要，但是对于其引诱蚜虫天敌的潜在分子机制并不清楚。

结果1-大灰优食蚜蝇受EBF引诱：Here, we first verified that the larvae and adults of aphid predator hoverfly Eupeodes corollae detect and are attracted to EBF.

本文中，作者首次证实了蚜虫捕食者大灰优食蚜蝇（Eupeodes corollae）能够发现并受到EBF的吸引。

结果2-神经元：Then, we found a neuron housed in type III basiconic sensilla of adult antenna responding to EBF.

接着，作者发现成年食蚜蝇触角的III型锥形感受器中的一个神经元对于EBF有响应。

结果3-气味蛋白：We further verified that in both adults and larvae odorant receptor EcorOR3 and odorant-binding protein EcorOBP15 mediate detection of EBF and structurally similar volatiles.

作者进一步证实了无论在食蚜蝇成体，还是在幼体中，其气味受体EcorOR3和气味结合蛋白EcorOBP15都会介导EBF及结构相似化合物的检测。

结果4-成体与幼体：Finally, we provide evidence that larvae of E. corollae may use aphid-derived EBF for prey location in the short-range, whereas adults could detect plant-derived EBF to find attacked plants from longer distances.

最后，作者发现大灰优食蚜蝇的幼体可能利用了源自于蚜虫的EBF来定位近处猎物，而大灰优食蚜蝇的成体能够检测到源自于植物的EBF来发现远处遭受蚜虫虫害的植物。

结论：Thus, while dissecting the molecular basis for attraction to EBF produced by two different sources, our results may find potential applications in integrated aphid management approaches.

因此，本文的研究解析了不同来源EBF引诱蚜虫天敌的分子基础，为未来综合治理植物蚜虫虫害提供了一个潜在解决方案。