郝兆东
the plant journal:油桐VfHB21基因作用于浓荫介导的种子油积累抑制
2021-10-16 12:37
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Triacylglycerol biosynthesis in shaded seeds of tung tree (Vernicia fordii) is regulated in part by Homeodomain Leucine Zipper 21

第一作者Lingling Zhang

第一单位武汉植物园

第一通讯Shiyou Lü


 Abstract 


背景回顾Light quantity and quality affect many aspects of plant growth and development. 


提出问题:However, few reports have addressed the molecular connections between seed oil accumulation and changes in light conditions, especially dense shade


研究背景:Shade-avoiding plants can redirect plant resources into extension growth at the expense of leaf and root expansion in an attempt to reach areas containing richer light.


主要研究:Here, we report that tung tree oil seed oil accumulation is suppressed by dense shade during the rapid oil accumulation phase. 


结果1-转录组分析:Transcriptome analysis confirmed that dense shade suppression of oil accumulation was attributed to reduced expression of fatty acid and triacylglycerol biosynthesis related genes


结果2-WGCNA:Through weighted gene co-expression network analysis, we identified 32 core transcription factors (TFs) specifically up-regulated in densely shaded seeds during rapid oil accumulation period. 


结果3-HD-ZIP基因HB21及其下游:Among these, VfHB21, a class I homeodomain leucine zipper TF, was shown to suppress expression of FAD2 and FADX, two key genes related to α-eleostearic acid by directly binding to HD-ZIP I/II motifs in their respective promoter regions. VfHB21 also binds to similar motifs in promoters of VfWRI1 and VfDGAT2, two additional key seed lipid regulatory/biosynthetic genes. 


结果4-基因功能验证:Functional conservation of HB21 during plant evolution was demonstrated by the fact that AtWRI1AtSAD1, and AtFAD2 were downregulated in VfHB21-overexpressor lines of transgenic Arabidopsis, with concomitant seed oil reduction, and that AtHB21 expression also was induced by shade. 


结论:This study reveals some of regulatory mechanisms that control tung tree seed oil biosynthesis specifically, and more broadly, plant storage carbon partitioning in response to dense shade conditions.


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 摘 要 


光量和光质影响着植物生长和发育的多个方面。但是,很少有报道探究种子油积累和光条件变化,尤其是浓荫条件之间的分子关联。避荫植物能够重新分配植物的资源,以牺牲叶片和根系扩张的代价,促进伸长生长,试图到达光线更丰富的位置。本文中,作者报道了桐树油籽中的油积累在其快速积累阶段会被浓荫所抑制。转录组分析显示,浓荫抑制油积累是通过降低脂肪酸和三酰甘油生物合成相关基因的表达。通过共表达网络分析,作者鉴定了32个核心转录因子在快速油积累期间,在浓荫处理的种子中特异性上调。其中,VfHB21是一种I类同源异型域-亮氨酸拉链蛋白(HD-Zip),通过直接结合到FAD2FADX基因的启动子区域中的HD-ZIP I/II基序,从而抑制这两个与α-榄香酸相关的关键基因的表达。VfHB21还能结合到另外两个关键的种子脂质调节/生物合成基因VFWRI1VfDGAT2的启动子区域的类似基序。在过表达VfHB21的转基因拟南芥中,AtWRI1AtSAD1AtFAD2基因的表达下调,同时种子的含油量减少,同时拟南芥AtHB21基因也会受到遮阴的诱导,说明HB21在植物演化过程中的功能比较保守。本文的研究结果揭示了一些控制桐树籽油生物合成的调控机制,以及植物贮藏碳分配对浓荫条件的响应。


 通讯作者 

** 吕世友 **


个人简介:

1994-1998年,解放军农牧大学,学士;

1998-2001年,解放军农牧大学,硕士;

2001-2004年,解放军军需大学,博士。


研究方向能源植物油桐高油脂新种质创新和可再生生物质能源开发


doi: https://doi.org/10.1111/tpj.15540


Journal: the plant journal

First PublishedOctober 13, 2021

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