Potassium transporter TRH1/KUP4 contributes to distinct auxin-mediated root system architecture responses
第一作者:Dimitris Templalexis
第一单位:雅典农业大学
第一通讯:Stamatis Rigas
Abstract
背景回顾:In plants, auxin transport and development are tightly coupled, just as hormone and growth responses are intimately linked in multicellular systems. 主要研究:Here we provide insights into uncoupling this tight control by specifically targeting the expression of TINY ROOT HAIR 1 (TRH1), a member of plant HAK/KUP/KT transporters that facilitate potassium uptake by co-transporting protons, in Arabidopsis root cell files. Use of this system pinpointed specific root developmental responses to acropetal versus basipetal auxin transport. 结果1-功能缺失:Loss of TRH1 function shows tinyroot hairs and defective root gravitropism, associated with auxin imbalance in the root apex. 结果2-功能回补:Cell file-specific expression of TRH1 in the central cylinder rescued trh1 root agravitropism, whereas positional TRH1 expression in peripheral cell layers, including epidermis and cortex, restored trh1 root hair defects. 结果3-根毛发育:Applying a systems-level approach, the role of RAP2.11 and RSL5 transcription factors in root hair development was verified. 结果4-根向重力性:Furthermore, ERF53 and WRKY51 transcription factors were overrepresented upon restoration of root gravitropism supporting involvement in gravitropic control. 结果5-生长素:Auxin has a central role in shaping root system architecture by regulating multiple developmental processes. 结论:We reveal that TRH1 jointly modulates intracellular ionic gradients and cell-to-cell polar auxin transport to drive root epidermal cell differentiation and gravitropic response. Our results indicate the developmental importance of HAK/KUP/KT proton-coupled K+ transporters. 摘 要
在植物中,生长素转运和发育是紧密相关的,正如激素与生长响应在多细胞系统中密切相关。本文中,作者通过在拟南芥根组织细胞中,特异性靶向植物中通过共转运质子促进钾离子吸收的HAK/KUP/KT转运蛋白编码基因TRH1,来为解偶联这种紧密关系提供新的见解。利用该系统,作者精确解析了植物根组织对于向顶和向基两个方向的生长素运输的特异性发育响应。TRH1基因功能缺失会导致根毛变得细小,并且根的向重力性出现缺陷,这与根尖中的生长素不平衡相关。通过在中柱细胞中特异性表达TRH1基因,能够拯救trh1突变体的根向重力性缺陷;而在表皮和皮层等根的外周细胞层中表达TRH1基因,能够拯救trh1突变体的根毛生长缺陷。利用一种系统水平的方法,作者验证了RAP2.11和RSL5转录因子在根毛发育中的作用。此外,一旦根的向重力性恢复,ERF53和WRKY51转录因子的表达即刻增加,说明其可能参与了根向重力性的控制。生长素通过调节多种发育过程,从而在根系结构的形成中发挥核心作用。作者发现TRH1共同调节细胞内离子梯度和细胞间极性生长素运输,以驱动根表皮细胞分化和向重力性响应。本文的研究结果表明HAK/KUP/KT质子偶联钾离子转运蛋白在植物发育过程中发挥重要作用。 通讯作者 ** Stamatis Rigas **
个人简介: 1990-1996年,雅典农业大学,学士; 1996-2001年,雅典农业大学,博士。 研究方向:调节细胞命运、植物器官形态发生和植物对内源信号或环境刺激的响应的分子网络。
doi: https://doi.org/10.1093/plphys/kiab472
Journal: Plant Physiology
Published date: October 11, 2021
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