A novel LRR-RLK (CTLK) confers cold tolerance through regulation on CBF pathway, antioxidants and proline accumulation
第一作者:Bohao Geng
第一单位:南京农业大学
第一通讯:Shaoyun Lu
Abstract
背景+问题:Leucine-rich repeat-receptor-like kinase (LRR-RLK) is a large subfamily of plant RLKs, however, its roles in cold tolerance remain unknown. 主要发现:A novel cold tolerance LRR-RLK gene (MtCTLK1) in Medicago truncatula was identified using the transgenic lines overexpressing MtCTLK1 (MtCTLK1-OE) and mtctlk1 lines with Tnt1 retrotransposon insertion. 结果1-过表达和敲除株系的抗寒表型:Compared to the wild type (WT), MtCTLK1-OE lines had increased cold tolerance and mtctlk1 showed decreased cold tolerance. 结果2-基因回补实验:The impaired cold tolerance in mtctlk1 could be complemented by the transgenic expression of MtCTLK1 or its homolog MfCTLK1 from Medicago falcata. 结果3-酶活、氨基酸测定:Antioxidant enzyme activities and proline accumulation as well as transcript levels of the associated genes were increased in response to cold, with higher levels in MtCTLK1-OE or lower levels in mtctlk1 lines as compared with WT. 结果4-CBF通路: C-Repeat-Binding Factors (CBFs) and CBF-dependent cold responsive genes were also induced in response to cold, and higher transcript levels of CBFs and CBF-dependent cold responsive genes were observed in MtCTLK1-OE lines whereas lower levels in mtctlk1mutants. 结论:The results validate the role of MtCTLK1 or MfCTLK1 in regulation of cold tolerance through CBF pathway, antioxidant defense system and proline accumulation. It also provides a valuable gene for the molecular breeding program to improve cold tolerance in crops. 摘 要
富含亮氨酸重复类受体激酶LRR-RLK是植物RLKs的一个大的亚家族,但是其在低温耐受性方面的作用还不清楚。本文中,作者通过过表达MtCTLK1和由Tnt1逆转座子插入获得的mtctlk1突变体,鉴定到了蒺藜苜蓿的一个新的低温耐受性LRR-RLK基因MtCTLK1。与野生型相比,MtCTLK1过表达株系的低温耐受性增强,而mtctlk1突变体的低温耐受性减弱。通过在mtctlk1突变体中表达MtCTLK1或者来自黄花苜蓿的同源基因MfCTLK1,能够拯救mtctlk1突变体的耐低温缺陷。与野生型相比,在寒冷条件下MtCTLK1过表达株系的抗氧化酶活性较高、脯氨酸积累较多、相关基因的转录水平增加,而mtctlk1突变体中则完全相反。CBFs和CBF依赖性的低温响应基因会受到低温的诱导,并且在MtCTLK1过表达株系中表达丰度更高,而在mtctlk1突变体中较低。本文的研究结果说明了MtCTLK1或者MfCTLK1通过CBF通路、抗氧化防御系统以及脯氨酸积累来作用于低温抗性。同时,本文的研究为未来作物的抗低温改良育种提供了一个候选靶基因。
通讯作者
** 卢少云 **
研究方向:草坪草抗逆育种。
doi: https://doi.org/10.1111/tpj.15535
Journal: the plant journal
First Published: October 09, 2021
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