郝兆东
Plant Cell:HBI介导的ROS稳态调控植物中的硝酸盐信号转导
2021-6-18 19:16
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HBI Transcription Factor-Mediated ROS Homeostasis Regulates Nitrate Signal Transduction

第一作者Xiaoqian Chu

第一单位山东大学

通讯作者Ming-Yi Bai


 Abstract 


背景回顾Nitrate is both an important nutrient and a critical signaling molecule that regulates plant metabolism, growth, and development. 


提出问题:Although several components of the nitrate signaling pathway have been identified, the molecular mechanism of nitrate signaling remains unclear. 


主要发现:Here, we showed that the growth-related transcription factors HOMOLOG OF BRASSINOSTEROID ENHANCED EXPRESSION2 INTERACTING WITH IBH1 (HBI1) and its three closest homologs (HBIs) positively regulate nitrate signaling in Arabidopsis thaliana


结果1-硝酸盐通过NLP6/7诱导HBI1HBI1 is rapidly induced by nitrate through NLP6 and NLP7, which are master regulators of nitrate signaling. 


结果2-HBI突变导致植物对硝酸盐响应减弱:Mutations in HBIs result in the reduced effects of nitrate on plant growth and approximately 22% nitrate-responsive genes no longer to be regulated by nitrate. 


结果3-HBI与ROSHBIs increase the expression levels of a set of antioxidant genes to reduce the accumulation of reactive oxygen species (ROS) in plants. 


结果4-HBI与CAT2/3突变干扰硝酸盐诱导的NLP7核定位Nitrate treatment induces the nuclear localization of NLP7, whereas such promoting effects of nitrate are significantly impaired in the hbi-q and cat2 cat3 mutants, which accumulate high levels of H2O2


结论1-HBI-ROS-NLP7-Nitrate signalling:These results demonstrate that HBI-mediated ROS homeostasis regulates nitrate signal transduction through modulating the nucleocytoplasmic shuttling of NLP7.


结论2-负反馈调节环:Overall, our findings reveal that nitrate treatment reduces the accumulation of H2O2, and H2Oinhibits nitrate signaling, thereby forming a feedback regulatory loop to regulate plant growth and development.


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 摘 要 


硝酸盐是一种重要的营养物质,也是调节植物代谢、生长和发育的重要信号分子。虽然,目前已经鉴定了硝酸盐信号途径上的几个组分,但硝酸盐信号转导的分子机制仍然不清楚。本文中,作者发现生长相关的转录因子HBI1及其三个最近缘的同源物(HBIs)相互作用,正调控拟南芥中的硝酸盐信号转导。硝酸盐通过NLP6和NLP7快速诱导HBI1,而NLP6和NLP7是硝酸盐信号转导的主要调控因子。HBIs的突变会导致硝酸盐对植物生长的影响减弱,约22%的硝酸盐响应基因不再受硝酸盐的诱导。HBIs通过增加一些抗氧化基因的表达,减少植物体内活性氧物质(ROS)的积累。硝酸盐处理会诱导NLP7的核定位,而hbi-qcat2 cat3突变体中硝酸盐的这种促进作用大大降低,并且这两种突变体植株体内均会积累较高水平的过氧化氢。这些结果表明,HBI介导的ROS稳态通过调节NLP7的核质穿梭,来调节硝酸盐的信号转导。综上,本文的研究结果表明,硝酸盐处理会减少了过氧化氢的积累,而过氧化氢则会抑制硝酸盐信号的传递,从而形成了一个反馈调节环来调节植物的生长和发育。


 通讯作者 

** 白明义 **


个人简介:

2007年,中科院植物所,博士。


研究方向植物激素油菜素内脂调控植物生长和碳-氮平衡利用的分子机理研究。


doi: https://doi.org/10.1093/plcell/koab165


Journal: Plant Cell

Published date: June 15, 2021

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