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第一作者:Hailin Liu
第一单位:南京林业大学
通讯作者:Fuliang Cao
Abstract
背景回顾:Gymnosperms are a unique lineage of plants that currently lack a high-quality reference genome due to their large genome size and high repetitive sequence content.
主要研究:Here, we report a nearly complete genome assembly for Ginkgo biloba with a genome size of 9.87 Gb, an N50 contig size of 1.58 Mb and an N50 scaffold size of 775 Mb.
结果1-基因组注释:We were able to accurately annotate 27,832 protein-coding genes in total, superseding the inaccurate annotation of 41,840 genes in a previous draft genome assembly.
结果2-基因组演化~大小:We found that expansion of the G. biloba genome, accompanied by the notable extension of introns, was mainly caused by the insertion of long terminal repeats rather than the recent occurrence of whole-genome duplication events, in contrast to the findings of a previous report.
结果3-银杏的精子鞭毛:We also identified candidate genes in the central pair, intraflagellar transport and dynein protein families that are associated with the formation of the spermatophore flagellum, which has been lost in all seed plants except ginkgo and cycads.
结论:The newly obtained Ginkgo genome provides new insights into the evolution of the gymnosperm genome.
摘 要
裸子植物是植物一个独特的支系,但由于其内植物的基因组较大、且富含较多的重复序列,所以目前仍然缺乏一个高质量的参考基因组。本文中,作者报道了一个银杏的高质量参考基因组,组装大小约9.87 Gb,contig N50达1.58Mb,scaffold N50长775Mb。作者共注释到了27832个蛋白编码基因,相比于之前的银杏基因组版本所注释的41,840个基因准确度大大提升。作者发现银杏基因组的扩张伴随着内含子的增长,这大多是由于长末端重复序列的插入导致的,而不是之前基因组版本所报道的最近发生的全基因组复制事件。作者还鉴定了与精子鞭毛形成相关的中心对蛋白、鞭毛内转运蛋白和动力蛋白家族,精子鞭毛这一性状在所有的种子植物中均已丢失,但在银杏和苏铁中还保留。该版本的银杏基因组组长将为裸子植物基因组的演化提供新的视野。
通讯作者
** 曹福亮 **
个人简介:
1978-1982年,南京林业大学,学士;
1986-1989年,南京林业大学,硕士;
1997-2004年,加拿大不列颠哥伦比亚大学,博士。
研究方向:重点开展银杏、杨树、落羽杉、毛竹等树种抗性生理、遗传育种、培育和加工,森林生态,森林文化等方面的研究。
doi: https://doi.org/10.1038/s41477-021-00933-x
Journal: Nature Plants
Published date: June 14, 2021
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