背景回顾：Mechanical stress influences cell- and tissue-scale processes across all kingdoms. 

提出问题：It remains challenging to delineate how mechanical stress, originating at these different length scales, impacts cell and tissue form. 

主要研究：We combine growth tracking of cells, quantitative image analysis, as well as molecular and mechanical perturbations to address this problem in pavement cells of Arabidopsis thaliana cotyledon tissue. 

结果1：We show that microtubule organization based on chemical signals and cell-shape-derived mechanical stress varies during early stages of pavement cell development and is mediated by the evolutionary conserved proteins, KATANIN and CLASP.

结果2：However, we find that these proteins regulate microtubule organization in response to tissue-scale mechanical stress to different extents in the cotyledon epidermis.

结果3：Our results further demonstrate that regulation of cotyledon form is uncoupled from the mechanical-stress-dependent control of pavement cell shape that relies on microtubule organization governed by subcellular mechanical stress.

 摘 要 

机械压力影响所有生物在细胞层面和组织层面上的生物学过程。在不同尺度层面上，理解机械压力如何影响细胞和组织形态仍然是一个挑战。本文中，作者基于拟南芥子叶铺板细胞，结合细胞生长追踪、定量图像分析、以及分子和机械扰动来研究该问题。作者发现，基于化学信号的微管组织和细胞形状衍生的机械压力在细胞发育早期阶段存在变异，并由演化上较为保守的蛋白KATANIN和CLASP所介导。然而，作者进一步发现在子叶表皮中，这些蛋白不同程度地调节微管组织对组织范围上机械压力的响应。作者的研究结果进一步表明，子叶形态的调控与机械压力依赖的铺板细胞形状控制是不耦合的，而后者依赖于由亚细胞层面机械压力影响的微管组织。