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第一作者:Hernán López
第一单位:马克斯普朗克植物育种研究所
通讯作者:Klaus Theres
Abstract
大背景:Shoot branching and complex leaf development rely on the establishment of boundaries that precedes the formation of axillary meristems and leaflets.
小背景:The tomato (Solanum lycopersicum) super determinant mutant is compromised in both processes, due to a mutation in Sde1A. Sde1A encodes a protein with a RAWUL domain, which is also present in Polycomb Group Repressive Complex 1 (PRC1) RING finger proteins and WD Repeat Domain 48 proteins.
结果1-遗传分析:Genetic analysis revealed that Sde1A and Bmi1A cooperate, whereas Bmi1C antagonizes both activities, indicating the existence of functionally opposing PRC1 complexes that interact with Sde1A.
结果2-表达模式与功能:Sde1A is expressed at early stages of boundary development in a small group of cells in the center of the leaf-axil boundary, but its activity is required for meristem formation at later stages. This suggests that Sde1A and Bmi1A promote axillary meristem formation and complex leaf development by safeguarding a pool of cells in the developing boundary zones.
结果3-Sde1A与LS:Genetic and protein interaction analyses showed that Sde1A and Lateral suppressor (Ls) are components of the same genetic pathway.
结论:In contrast to ls, sde1a mutants are not compromised in inflorescence branching, suggesting that Sde1A is a potential target for breeding tomato cultivars with reduced side-shoot formation during vegetative development.
摘 要
茎分枝和复叶的发育依赖于腋生分生组织和小叶形成之前边界的建立。番茄super determinant突变体在这两个生物学过程方面均存在缺陷,而该突变体突变的基因是Sde1A。Sde1A基因编码一个含有RAWUL结构域的蛋白,这种结构域也存在于多梳蛋白抑制复合体(PRC1)RING finger蛋白和WD Repeat Domain 48蛋白中。遗传分析显示,Sde1A和Bmi1A协作,而Bmi1C拮抗这两个基因的活性,表明存在与Sde1A互作、与PRC1功能相反的复合物。Sde1A在叶腋边界中心早期边界发育过程中的一小群细胞中表达,但其对于后期分生组织的形成是必需的。这表明Sde1A和Bmi1A通过保护正在发育中的边界区细胞池,来促进腋生分生组织的形成和复叶的发育。遗传和蛋白互作分析表明,Sde1A和Lateral suppressor是同一遗传途径上的组分。与ls突变体相比,sde1a突变体在花序分枝方面没有受到影响,这表明sde1a是培育营养发育过程中侧枝形成减少的番茄品种的一个潜在靶标。
doi: https://doi.org/10.1093/plcell/koab121
Journal: Plant Cell
Published date: May 01, 2021
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