Vascular bundles mediate systemic reactive oxygen signaling during light stress in Arabidopsis
第一作者:Sara I Zandalinas
第一单位:密苏里大学
通讯作者:Ron Mittler
Abstract
大背景:Systemic signaling and systemic acquired acclimation (SAA) are essential for plant survival during episodes of environmental stress.
小背景:Recent studies highlighted a key role for reactive oxygen species (ROS) signaling in mediating systemic responses and SAA during light stress in Arabidopsis thaliana. These studies further identified the RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) protein as a key player in mediating rapid systemic ROS responses.
提出问题:However, the identity of the plant tissues and cells that transmit systemic ROS signals in plants, as well as the identity of other ROS-producing proteins involved in this process are currently unknown.
结果1-RBOHD:Here, we report that tissue-specific expression of RBOHD in phloem or xylem parenchyma cells of the rbohD mutant restores systemic ROS signaling, systemic stress-response transcript expression and SAA to a local treatment of light stress.
结果2-RBOHD/F:We further demonstrate that RBOHD and RBOHF are both required for local and systemic ROS signaling at the vascular bundlesof Arabidopsis.
结论1:Taken together our findings highlight a key role for RBOHD-driven ROS production at the vascular bundles of Arabidopsis in mediating light stress-induced systemic signaling and SAA.
结论2:In addition, they suggest that the integration of ROS, calcium, electric and hydraulic signals, during systemic signaling, occurs at the vascular bundles.
摘 要
系统性信号和系统获得性适应(SAA)对于植物在不同环境胁迫下的存活是必要的。最近的研究显示,在拟南芥中,光胁迫下由活性氧物质ROS信号介导的系统性响应和SAA发挥了非常关键的作用。这些研究进一步鉴定了呼吸爆发氧化酶同系物蛋白D(RBOHD)是介导快速系统性ROS响应的关键因子。然而,植物中参与系统性ROS信号转导的植物组织和细胞还不清楚,同时参与ROS产生的其他蛋白也不清晰。本文中,作者发现RBOHD在rbohD突变体植株中的韧皮部或木质部薄壁细胞特异性表达能够恢复植株对于局部的光胁迫处理所产生的系统性ROS信号、系统性胁迫响应转录本表达以及SAA。作者进一步发现拟南芥中,RBOHD和RBOHF对于维管束中局部和系统性的ROS信号是必须的。综上,作者的研究显示拟南芥中,RBOHD驱动的ROS在维管束中的生成对于光胁迫诱导的系统性信号和SAA都是非常重要的。另外,本文的研究还表明在系统性信号转导过程中,植物的维管束同时整合了ROS信号、钙信号、电信号以及水力液压信号。
通讯作者
**Ron Mittler**
个人简介:
耶路撒冷希伯来大学,学士;
耶路撒冷希伯来大学,硕士;
新泽西州罗格斯大学,博士。
研究方向:
ROS在植物生长发育中的作用。
doi: 10.1105/tpc.20.00453
Journal: Plant Cell
First Published: September 15, 2020
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