A Self-Organized PLT/Auxin/ARR-B Network Controls the Dynamics of Root Zonation Development in Arabidopsis thaliana
第一作者:Elena Salvi
第一单位:意大利罗马大学
通讯作者:Kirsten Ten Tusscher
Abstract
背景回顾:During organogenesis, coherent organ growth arises from spatiotemporally coordinated decisions of individual cells. In the root of Arabidopsis thaliana, this coordination results in the establishment of a division and a differentiation zone.
提出问题:Cells continuously move through these zones; thus, a major question is how the boundary between these domains, the transition zone, is formed and maintained.
主要发现:By combining molecular genetics with computational modeling, we reveal how an auxin/PLETHORA/ARR-B network controls these dynamic patterning processes.
结果:We show that after germination, cell division causes a drop in distal PLT2 levels that enables transition zone formation and ARR12 activation. The resulting PLT2-ARR12 antagonism controls expansion of the division zone (the meristem). The successive ARR1 activation antagonizes PLT2 through inducing the cell-cycle repressor KRP2, thus setting final meristem size.
结论:Our work indicates a key role for the interplay between cell division dynamics and regulatory networks in root zonation and transition zone patterning.
摘 要
在器官发生过程中,相连器官的生长源自于单个细胞在时空上的协调决策。在拟南芥的根中,这种协调性建立起了分裂区(division zone)和分化区(differentiation zone)。细胞在这些区域间连续移动,所以一个关键的问题就是这些区域的界限是如何确定的,以及过渡区(transition zone)是如何形成及保持的。通过结合分子遗传和计算建模,作者在本文中揭示了一个生长素/PLETHORA/ARR-B网络是如何控制这些动态的模式进程的。作者发现,自种子萌发后,细胞分裂会导致远端的PLT2水平下降,从而保证过渡区的形成及ARR12的激活。而PLT2-ARR12的拮抗控制了分裂区,即分生组织的扩张。连续的ARR1激活通过诱导细胞周期抑制基因KRP2来拮抗PLT2,从而最终决定分生组织的大小。本文的研究揭示了拟南芥根分区和过渡区模式建成过程中细胞分裂动态与调控网络之间互作的关键作用。
Highlights
Upon germination, cell divisions generate a PLT drop that forms the transition zone
拟南芥种子一旦萌发后,细胞分裂会产生PLT水平下降,从而形成过渡区
A PLTs-ARR12 mutual antagonism restricts early root meristem expansion
PLTs与ARR12之间的相互拮抗,限制了根分生组织的早期扩张
ARR1 repression of cell division via KRP2 is key for meristem size stabilization
ARR1通过KRP2抑制细胞分裂对于保持分生组织大小稳定十分重要
Auxin-PLTs-ARRs form a network responsible for self-organized root patterning
Auxin-PLTs-ARRs形成了一个网络,作用于自组织的根模式建成
通讯作者
**Kirsten Ten Tusscher**
研究方向:基于从亚细胞到整个生物体层面收集的生物数据,
利用复杂的计算模拟,
揭示多细胞生物发育过程中的复杂模式建成。
doi: 10.1016/j.devcel.2020.04.004
Journal: Developmental Cell
Published date: May 07, 2020
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