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受人口增长、环境污染、气候变化等因素影响,我国蛋白资源的日常供应正面临着严峻的挑战。威尼斯镰刀菌发酵生产的菌丝体蛋白,由于具有安全性好、营养价值高、生产周期短、可连续生产等优点,是一种能够满足于现代人营养需求且具有广阔应用前景的肉类代用品。为了进一步提高威尼斯镰刀菌菌丝体蛋白发酵特性,课题组前期已在TB01菌株中成功建立稳定的遗传转化体系(Tong et al., Journal of Fungi, 2022)和高效的DNA-Free CRISPR/Cas9编辑体系(Tong et al., Applied Microbiology and Biotechnology, 2022)。上述体系的建立可以实现靶基因的高效敲除,然而如果要确保靶基因的定向插入(不破坏基因组内关键基因)且高效、稳定的表达,则还需要提供理想的中性基因组整合位点。
近日,天津工生所李德茂团队在威尼斯镰刀菌TB01中鉴定了两个中性基因组整合位点,在CRISPR/Cas9介导的同源定向重组下可以实现靶标基因100%定向整合并稳定高效表达。相关研究成果以“Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01”为题发表在 Synthetic and Systems Biotechnology 期刊上。
https://blog.sciencenet.cn/blog-3496796-1372293.html
http://www.pubmedplus.cn/P/SearchQuickResult?wd=066e4fcb-22bd-4d8c-9e68-9af887934893
01. | 2022 | 2 篇 | 66.667% |
02. | 2017 | 1 篇 | 33.333% |
01. | biology (basel) | 1 篇 | 33.333% |
02. | j biotechnol | 1 篇 | 33.333% |
03. | j gen appl microbiol | 1 篇 | 33.333% |
01. | 中国 | 2 篇 | 66.667% |
02. | 日本 | 1 篇 | 33.333% |
01. | 中国北京 | 1 篇 | 33.333% |
02. | 中国天津 | 1 篇 | 33.333% |
03. | 中国上海 | 1 篇 | 33.333% |
04. | 中国临沂 | 1 篇 | 33.333% |
01. | Acremonium | 1 篇 | 33.333% |
02. | Aspergillus oryzae | 1 篇 | 33.333% |
03. | CRISPR-Cas Systems | 1 篇 | 33.333% |
04. | Cephalosporins | 1 篇 | 33.333% |
05. | Fungal Proteins | 1 篇 | 33.333% |
06. | Gene Deletion | 1 篇 | 33.333% |
07. | Gene Editing | 1 篇 | 33.333% |
08. | Gene Expression Regulation, Fungal | 1 篇 | 33.333% |
09. | Gene Targeting | 1 篇 | 33.333% |
10. | Hyphae | 1 篇 | 33.333% |
http://www.pubmedplus.cn/P/SearchQuickResult?wd=271db1f0-cd08-4bd0-8304-ec0bc98e86cd
01. | Fermentation | 49 篇 | 42.241% |
02. | Mycelium | 39 篇 | 33.621% |
03. | Fungal Proteins | 38 篇 | 32.759% |
04. | Hyphae | 25 篇 | 21.552% |
05. | Culture Media | 19 篇 | 16.379% |
06. | Bacterial Proteins | 14 篇 | 12.069% |
07. | Molecular Sequence Data | 13 篇 | 11.207% |
08. | Animals | 12 篇 | 10.345% |
09. | Aspergillus niger | 12 篇 | 10.345% |
10. | Humans | 12 篇 | 10.345% |
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