标题：Target specificity of the CRISPR-Cas9 system

这篇文章是来自菲利普·夏普（Phillip Allen Sharp）的实验室，他是本文的通讯作者，Phillip A. Sharp是美国遗传学家与分子生物学家，1993年发现断裂基因而获得诺贝尔生理学奖

文章摘要

The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided

DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown

great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these

applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.

详细内容请访问高等教育出版社学术前沿期刊网http://journal.hep.com.cn/qb/EN/10.1007/s40484-014-0030-x