小柯机器人

以色列特拉维夫大学Roy Weinstain和以色列农业和农村发展部Einat Sadot共同合作，近期取得重要工作进展。他们研究提出，合成生长素的缓慢释放能够诱导顽固性木本植物不定根的形成。相关研究成果2024年1月24日在线发表于《自然—生物技术》杂志上。

据介绍，从茎插条诱导不定根（AR）进行植物无性繁殖是育种计划中的必要步骤。繁殖有价值的植物存在一个主要障碍，这些植物自然形成AR的能力很低。由于生长素在器官发生中的核心作用，吲哚-3-丁酸经常被用作商业生根混合物的一部分，但许多顽固性植物对这种处理没有形成AR。

研究人员描述了在大桉树插条中合成生长素偶联物文库的合成和筛选，并确定4-氯苯氧乙酸-L-色氨酸-OMe是许多顽固木本植物（包括苹果和爱尔兰坚果）不定根的有效增强剂。综合代谢和功能分析表明，这种活性是由生长素信号传导的延长引起的，生长素信号传导是由于游离生长素4-氯苯氧乙酸的初始快速吸收、缓慢释放和清除。

总之，这项工作强调了生物活性化合物的缓释策略对更有效的植物生长调控的效用。

附：英文原文

Title: Slow release of a synthetic auxin induces formation of adventitious roots in recalcitrant woody plants

Author: Roth, Ohad, Yechezkel, Sela, Serero, Ori, Eliyahu, Avi, Vints, Inna, Tzeela, Pan, Carignano, Alberto, Janacek, Dorina P., Peters, Verena, Kessel, Amit, Dwivedi, Vikas, Carmeli-Weissberg, Mira, Shaya, Felix, Faigenboim-Doron, Adi, Ung, Kien Lam, Pedersen, Bjrn Panyella, Riov, Joseph, Klavins, Eric, Dawid, Corinna, Hammes, Ulrich Z., Ben-Tal, Nir, Napier, Richard, Sadot, Einat, Weinstain, Roy

Issue&Volume: 2024-01-24

Abstract: Clonal propagation of plants by induction of adventitious roots (ARs) from stem cuttings is a requisite step in breeding programs. A major barrier exists for propagating valuable plants that naturally have low capacity to form ARs. Due to the central role of auxin in organogenesis, indole-3-butyric acid is often used as part of commercial rooting mixtures, yet many recalcitrant plants do not form ARs in response to this treatment. Here we describe the synthesis and screening of a focused library of synthetic auxin conjugates in Eucalyptus grandis cuttings and identify 4-chlorophenoxyacetic acid–L-tryptophan-OMe as a competent enhancer of adventitious rooting in a number of recalcitrant woody plants, including apple and argan. Comprehensive metabolic and functional analyses reveal that this activity is engendered by prolonged auxin signaling due to initial fast uptake and slow release and clearance of the free auxin 4-chlorophenoxyacetic acid. This work highlights the utility of a slow-release strategy for bioactive compounds for more effective plant growth regulation.

DOI: 10.1038/s41587-023-02065-3

Source: https://www.nature.com/articles/s41587-023-02065-3

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex