小柯机器人

英国东英吉利大学Jonathan D. G. Jones、Xiao Lin、中国农业科学院Sanwen Huang和韩国浦项科技大学Kee Hoon Sohn研究组，合作利用少花龙葵的免疫受体基因组发现了马铃薯晚疫病的病原体效应因子。相关论文于2023年8月28日发表于国际学术期刊《自然-遗传学》杂志。

研究人员揭示了四个少花龙葵种质的高质量参考基因组，对52个种质进行了重新测序，并确定了少花龙葵免疫受体基因的泛NLRome。研究人员进一步筛选了52个少花龙葵种质中315P感染者RXLR效应因子的识别差异。

利用这些基因组和表型数据，研究人员克隆了三个NLR编码基因Rpi-amr4、R02860和R04373，它们分别识别同源P. infestans RXLR的效应因子PITG_22825（AVRamr4）、PITG_02860和PITG_04373。这些基因组资源和方法将支持培育具有持久抗晚疫病的马铃薯，并可应用于其他抗疾病作物的研发。

据了解，马铃薯（Solanum tuberosum）和番茄（Solanum lycopersicon）作物因受致病疫霉引发的晚疫病而遭受严重损失。少花龙葵是马铃薯和番茄的近亲，分布于全球，大多数品种具有高度抗枯萎病特性。

附：英文原文

Title: Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors

Author: Lin, Xiao, Jia, Yuxin, Heal, Robert, Prokchorchik, Maxim, Sindalovskaya, Maria, Olave-Achury, Andrea, Makechemu, Moffat, Fairhead, Sebastian, Noureen, Azka, Heo, Jung, Witek, Kamil, Smoker, Matthew, Taylor, Jodie, Shrestha, Ram-Krishna, Lee, Yoonyoung, Zhang, Chunzhi, Park, Soon Ju, Sohn, Kee Hoon, Huang, Sanwen, Jones, Jonathan D. G.

Issue&Volume: 2023-08-28

Abstract: Potato (Solanum tuberosum) and tomato (Solanum lycopersicon) crops suffer severe losses to late blight caused by the oomycete pathogen Phytophthora infestans. Solanum americanum, a relative of potato and tomato, is globally distributed and most accessions are highly blight resistant. We generated high-quality reference genomes of four S. americanum accessions, resequenced 52 accessions, and defined a pan-NLRome of S. americanum immune receptor genes. We further screened for variation in recognition of 315P. infestans RXLR effectors in 52 S. americanum accessions. Using these genomic and phenotypic data, we cloned three NLR-encoding genes, Rpi-amr4, R02860 and R04373, that recognize cognate P. infestans RXLR effectors PITG_22825 (AVRamr4), PITG_02860 and PITG_04373. These genomic resources and methodologies will support efforts to engineer potatoes with durable late blight resistance and can be applied to diseases of other crops.

DOI: 10.1038/s41588-023-01486-9

Source: https://www.nature.com/articles/s41588-023-01486-9

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