小柯机器人

美国波士顿大学Darrell N. Kotton研究小组取得一项新突破。他们通过移植原代或多能干细胞（PSC）来源的基底细胞（BC）完成了气道干细胞重建。相关论文于2023年8月24日发表在《细胞-干细胞》杂志上。

研究人员通过气道内移植小鼠或人原代多能干细胞来源的气道基底细胞，实现了气道上皮干细胞室的重建。将小鼠原代或PSC来源的BCs移植到聚多卡醇损伤的同系受体中，产生的子代细胞至少能存在两年，并具有稳定的气道上皮形态、分子和功能表型。移植的基底样细胞保留了多样的自我更新潜力，并通过7代传代细胞的二次移植重建气管上皮证明了这一点。

使用相同的方法，移植到NOD scid γ（NSG）受体小鼠中的人原代或PSC衍生的BC在体内同样具有多谱系气道上皮分化能力。该研究结果可能为患有气道上皮细胞损伤或上皮细胞功能障碍疾病的患者，提供潜在的基于同源细胞的治疗方法。

研究人员表示，用移植细胞实现组织常驻干细胞区室终生重建可能会持久维持器官功能。

附：英文原文

Title: Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells

Author: Liang Ma, Bibek R. Thapa, Jake A. Le Suer, Andrew Tilston-Lünel, Michael J. Herriges, Andrew Berical, Mary Lou Beermann, Feiya Wang, Pushpinder S. Bawa, Anat Kohn, Alexandra B. Ysasi, Hirofumi Kiyokawa, Taylor M. Matte, Scott H. Randell, Xaralabos Varelas, Finn J. Hawkins, Darrell N. Kotton

Issue&Volume: 2023-08-24

Abstract: Life-long reconstitution of a tissue’s resident stem cell compartment with engrafted cells has the potential to durably replenish organ function. Here, we demonstrate the engraftment of the airway epithelial stem cell compartment via intra-airway transplantation of mouse or human primary and pluripotent stem cell (PSC)-derived airway basal cells (BCs). Murine primary or PSC-derived BCs transplanted into polidocanol-injured syngeneic recipients give rise for at least two years to progeny that stably display the morphologic, molecular, and functional phenotypes of airway epithelia. The engrafted basal-like cells retain extensive self-renewal potential, evident by the capacity to reconstitute the tracheal epithelium through seven generations of secondary transplantation. Using the same approach, human primary or PSC-derived BCs transplanted into NOD scid gamma (NSG) recipient mice similarly display multilineage airway epithelial differentiation in vivo. Our results may provide a step toward potential future syngeneic cell-based therapy for patients with diseases resulting from airway epithelial cell damage or dysfunction.

DOI: 10.1016/j.stem.2023.07.014

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(23)00281-3

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