小柯机器人

中国科学院王晓群、吴倩和王拥军研究组报道了人类原肠胚形成和早期大脑发育的单细胞和空间转录景观。相关论文发表在2023年5月15日出版的《细胞—干细胞》杂志上。

三种生殖层的出现和协调器官发生的谱系特异性前体细胞是早期胚胎发育的重要里程碑。他们分析了14个人类样本中超过400,000个细胞的转录谱，这些样本收集于受孕后第3周至第12周，以描绘早期原肠胚形成和神经系统发育的动态分子和细胞景观。他们描述了细胞类型的多样化，神经管细胞的空间模式，以及可能参与将外胚层细胞转化为神经上皮细胞，然后再转化为放射状胶质细胞的信号通路。

他们解析了沿神经管排列的24簇放射状胶质细胞，并勾勒出主要神经元类别的分化轨迹。最后，他们通过比较人类和小鼠的早期胚胎单细胞转录组谱，确定了物种间的保守和独特特征。这个全面的图谱揭示了原肠胚形成和早期人类大脑发育的分子机制。

附：英文原文

Title: The single-cell and spatial transcriptional landscape of human gastrulation and early brain development

Author: Bo Zeng, Zeyuan Liu, Yufeng Lu, Suijuan Zhong, Shenyue Qin, Luwei Huang, Yang Zeng, Zixiao Li, Hao Dong, Yingchao Shi, Jialei Yang, Yalun Dai, Qiang Ma, Le Sun, Lihong Bian, Dan Han, Youqiao Chen, Xin Qiu, Wei Wang, Oscar Marín, Qian Wu, Yongjun Wang, Xiaoqun Wang

Issue&Volume: 2023-05-15

Abstract: The emergence of the three germ layers and the lineage-specific precursor cells orchestrating organogenesis represent fundamental milestones during early embryonic development. We analyzed the transcriptional profiles of over 400,000 cells from 14 human samples collected from post-conceptional weeks (PCW) 3 to 12 to delineate the dynamic molecular and cellular landscape of early gastrulation and nervous system development. We described the diversification of cell types, the spatial patterning of neural tube cells, and the signaling pathways likely involved in transforming epiblast cells into neuroepithelial cells and then into radial glia. We resolved 24 clusters of radial glial cells along the neural tube and outlined differentiation trajectories for the main classes of neurons. Lastly, we identified conserved and distinctive features across species by comparing early embryonic single-cell transcriptomic profiles between humans and mice. This comprehensive atlas sheds light on the molecular mechanisms underlying gastrulation and early human brain development.

DOI: 10.1016/j.stem.2023.04.016

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(23)00134-0

Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
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