小柯机器人

中国科学院脑科学与智能技术卓越创新中心严军、徐宁龙和华中科技大学Hui Gong研究组合作取得一项新成果。经过不懈努力，他们描绘了小鼠前额叶单神经元投射图谱。2022年3月31日出版的《自然-神经科学》发表了这项成果。

通过对小鼠前额叶皮层(PFC)6,357个投射神经元的单神经元重建，研究人员确定了64个投射组的亚型。四个先前已知的主要皮质-皮质子网络中的每一个亚型都对应于一组不同的PFC亚型，这些亚型通过它们的一阶轴突侧枝确定。进一步分析揭示了PFC内体细胞分布的位置规则、一阶侧枝分支点依赖的目标选择和末端分布依赖的目标细分。

此外，研究人员在PFC和三个功能不同相关PFC模块中获得了高精度的层次图，每个模块都富含内部循环连接。最后，研究发现每个转录组亚型对应于在不同PFC亚区中发现的多个目标亚型。因此，全脑单神经元投射图分析揭示了PFC内外轴突投射的组织原理，为阐明不同PFC功能背后的神经元连接提供了基础依据。

据悉，PFC是整合和调节大脑整体活动的认知中心。然而，对PFC轴突在全脑组织内的投射仍知之甚少。

附：英文原文

Title: Single-neuron projectome of mouse prefrontal cortex

Author: Gao, Le, Liu, Sang, Gou, Lingfeng, Hu, Yachuang, Liu, Yanhe, Deng, Li, Ma, Danyi, Wang, Haifang, Yang, Qiaoqiao, Chen, Zhaoqin, Liu, Dechen, Qiu, Shou, Wang, Xiaofei, Wang, Danying, Wang, Xinran, Ren, Biyu, Liu, Qingxu, Chen, Tianzhi, Shi, Xiaoxue, Yao, Haishan, Xu, Chun, Li, Chengyu T., Sun, Yangang, Li, Anan, Luo, Qingming, Gong, Hui, Xu, Ninglong, Yan, Jun

Issue&Volume: 2022-03-31

Abstract: Prefrontal cortex (PFC) is the cognitive center that integrates and regulates global brain activity. However, the whole-brain organization of PFC axon projections remains poorly understood. Using single-neuron reconstruction of 6,357 mouse PFC projection neurons, we identified 64 projectome-defined subtypes. Each of four previously known major cortico-cortical subnetworks was targeted by a distinct group of PFC subtypes defined by their first-order axon collaterals. Further analysis unraveled topographic rules of soma distribution within PFC, first-order collateral branch point-dependent target selection and terminal arbor distribution-dependent target subdivision. Furthermore, we obtained a high-precision hierarchical map within PFC and three distinct functionally related PFC modules, each enriched with internal recurrent connectivity. Finally, we showed that each transcriptome subtype corresponds to multiple projectome subtypes found in different PFC subregions. Thus, whole-brain single-neuron projectome analysis reveals organization principles of axon projections within and outside PFC and provides the essential basis for elucidating neuronal connectivity underlying diverse PFC functions. The authors reconstructed the individual projectomes of 6,357 mouse prefrontal cortical projection neurons, revealing projectome-defined neuron subtypes and organizing principles of axon projections and correspondence with transcriptomes.

DOI: 10.1038/s41593-022-01041-5

Source: https://www.nature.com/articles/s41593-022-01041-5

Nature Neuroscience：《自然—神经科学》，创刊于1998年。隶属于施普林格·自然出版集团，最新IF：28.771
官方网址：https://www.nature.com/neuro/
投稿链接：https://mts-nn.nature.com/cgi-bin/main.plex