小柯机器人

美国麻省理工学院Alan Jasanoff小组利用fMRI的遗传报告器实现神经回路的功能剖析。2022年3月3日，《自然—神经科学》杂志在线发表了这项成果。

Author: Ghosh, Souparno, Li, Nan, Schwalm, Miriam, Bartelle, Benjamin B., Xie, Tianshu, Daher, Jade I., Singh, Urvashi D., Xie, Katherine, DiNapoli, Nicholas, Evans, Nicholas B., Chung, Kwanghun, Jasanoff, Alan

Issue&Volume: 2022-03-03

Abstract: The complex connectivity of the mammalian brain underlies its function, but understanding how interconnected brain regions interact in neural processing remains a formidable challenge. Here we address this problem by introducing a genetic probe that permits selective functional imaging of distributed neural populations defined by viral labeling techniques. The probe is an engineered enzyme that transduces cytosolic calcium dynamics of probe-expressing cells into localized hemodynamic responses that can be specifically visualized by functional magnetic resonance imaging. Using a viral vector that undergoes retrograde transport, we apply the probe to characterize a brain-wide network of presynaptic inputs to the striatum activated in a deep brain stimulation paradigm in rats. The results reveal engagement of surprisingly diverse projection sources and inform an integrated model of striatal function relevant to reward behavior and therapeutic neurostimulation approaches. Our work thus establishes a strategy for mechanistic analysis of multiregional neural systems in the mammalian brain. This study introduces genetically encoded imaging probes that convert intracellular calcium signaling into hemodynamic fMRI responses. The authors show how the probes can be used to map information flow in reward-related brain circuitry in rats.

DOI: 10.1038/s41593-022-01014-8

Source: https://www.nature.com/articles/s41593-022-01014-8

Nature Neuroscience：《自然—神经科学》，创刊于1998年。隶属于施普林格·自然出版集团，最新IF：28.771
官方网址：https://www.nature.com/neuro/
投稿链接：https://mts-nn.nature.com/cgi-bin/main.plex