小柯机器人

英国剑桥大学Wolf Reik、Jasmin Taubenschmid-Stowers等研究人员合作发现，8C样细胞可在体外捕获人类合子基因组激活程序。2022年2月24日，《细胞—干细胞》杂志在线发表了这项成果。

研究人员在初始胚胎干细胞中发现了人类8细胞样细胞（8CLC），它们在转录上类似于人类8细胞胚胎。它们表达合子基因组激活（ZGA）标记，包括ZSCAN4和LEUTX，以及可转座元件，如HERVL和MLT2A1。8CLC显示出SOX2水平的降低，并可在体外使用TPRX1和H3.Y标记蛋白进行鉴定。转录因子DUX4的过量表达和剪接体的抑制增加了人类ZGA样转录。令人兴奋的是，8CLC标志物TPRX1和H3.Y也在ZGA阶段的人类胚胎中表达，因此可能与体内有关。8CLC提供了一个独特的机会来描述人类ZGA样转录的特征，并可能提供对人类胚胎发育早期事件的关键性见解。

据悉，胚胎基因组的激活标志着发育中的生物体的第一波主要转录。小鼠2细胞胚胎和人类8细胞胚胎中的ZGA对发育至关重要。

附：英文原文

Title: 8C-like cells capture the human zygotic genome activation program in vitro

Author: Jasmin Taubenschmid-Stowers, Maria Rostovskaya, Fátima Santos, Sebastian Ljung, Ricard Argelaguet, Felix Krueger, Jennifer Nichols, Wolf Reik

Issue&Volume: 2022-02-24

Abstract: The activation of the embryonic genome marks the first major wave of transcription in the developing organism. Zygotic genome activation (ZGA) in mouse 2-cell embryos and 8-cell embryos in humans is crucial for development. Here, we report the discovery of human 8-cell-like cells (8CLCs) among naive embryonic stem cells, which transcriptionally resemble the 8-cell human embryo. They express ZGA markers, including ZSCAN4 and LEUTX, and transposable elements, such as HERVL and MLT2A1. 8CLCs show reduced SOX2 levels and can be identified using TPRX1 and H3.Y marker proteins in vitro. Overexpression of the transcription factor DUX4 and spliceosome inhibition increase human ZGA-like transcription. Excitingly, the 8CLC markers TPRX1 and H3.Y are also expressed in ZGA-stage 8-cell human embryos and may thus be relevant in vivo. 8CLCs provide a unique opportunity to characterize human ZGA-like transcription and might provide critical insights into early events in embryogenesis in humans.

DOI: 10.1016/j.stem.2022.01.014

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00050-9

Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
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