小柯机器人

中山大学中山医学院王继厂研究团队发现化学诱导的染色质重塑可将小鼠 ESC 重编程为全能样干细胞 (TLSCs)。相关论文于2022年2月9日发表在《细胞—干细胞》杂志上。

他们重塑着丝粒周围异染色质并重建全能特异性宽 H3K4me3 结构域的方法促进了多能性向全能性的转变。他们的方法建立了比任何其他 2C 样细胞更紧密的小鼠 2 细胞 (2C) 胚胎匹配。这些TLSC在培养中是稳定的，并具有小鼠 2C 胚胎的独特分子特征。在功能上，TLSCs 能够进行种系传播，并以高频率产生胚胎和胚胎外谱系。因此，TLSCs 代表了一种非常有价值的细胞类型，用于研究全能性和胚胎学。

据悉，全能细胞比任何其他细胞类型具有更强的发育潜能，从而产生胚胎和胚胎外组织。稳定的全能细胞培养和破译全能性调控的原理对于了解早期发育中的细胞可塑性和谱系分离是非常宝贵的。

附：英文原文

Title: Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells

Author: Mingzhu Yang, Hanwen Yu, Xiu Yu, Shiqi Liang, Yuanlang Hu, Yuxin Luo, Zsuzsanna Izsvák, Chuanbo Sun, Jichang Wang

Issue&Volume: 2022-02-09

Abstract: Totipotent cells have more robust developmental potency than any other cell types,giving rise to both embryonic and extraembryonic tissues. Stable totipotent cell culturesand deciphering the principles of totipotency regulation would be invaluable to understandcell plasticity and lineage segregation in early development. Our approach of remodelingthe pericentromeric heterochromatin and re-establishing the totipotency-specific broadH3K4me3 domains promotes the pluri-to-totipotency transition. Our protocol establishesa closer match of mouse 2-cell (2C) embryos than any other 2C-like cells. These totipotent-likestem cells (TLSCs) are stable in culture and possess unique molecular features ofthe mouse 2C embryo. Functionally, TLSCs are competent for germline transmission andgive rise to both embryonic and extraembryonic lineages at high frequency. Therefore,TLSCs represent a highly valuable cell type for studies of totipotency and embryology.

DOI: 10.1016/j.stem.2022.01.010

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00010-8

Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
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