加拿大多伦多大学Tae-Hee Kim研究组发现肠神经胶质细胞 (EGC)异质性可调节肠道干细胞壁龛。该研究于2021年11月1日发表于国际一流学术期刊《细胞—干细胞》杂志上。
通过在单细胞水平分析小鼠和人肠粘膜转录组，他们定义了EGC异质性在体内平衡和慢性炎症性肠病中的调节。EGC 亚群的消融表明，肠干细胞 (ISC) 的修复潜力受特定的胶质纤维酸性蛋白 (GFAP) + EGC 亚群的调节。
从机制上讲，损伤会诱导 GFAP+ EGC 的扩张，其表达几种 WNT 配体以促进 LGR5+ ISC自我更新。他们的工作揭示了作为肠道干细胞壁龛在再生和疾病中的关键部分的 EGC 的动态调节异质性。
据悉，成人肠道的高周转和再生能力依赖于位于隐窝底部的常驻干细胞。肠神经系统由丰富的EGC和神经元网络组成。尽管 EGC 与干细胞非常接近，但它们作为干细胞壁龛的体内作用仍不清楚。
附：英文原文
Title: Enteric glial cell heterogeneity regulates intestinal stem cell niches
Author: Meryem B. Baghdadi, Arshad Ayyaz, Sabrina Coquenlorge, Bonnie Chu, Sandeep Kumar, Catherine Streutker, Jeffrey L. Wrana, Tae-Hee Kim
Issue&Volume: 2021-11-01
Abstract: The high turnover and regenerative capacity of the adult intestine relies on residentstem cells located at the bottom of the crypt. The enteric nervous system consistsof an abundant network of enteric glial cells (EGCs) and neurons. Despite the closeproximity of EGCs to stem cells, their in vivo role as a stem cell niche is still unclear. By analyzing the mouse and human intestinalmucosa transcriptomes at the single-cell level, we defined the regulation of EGC heterogeneityin homeostasis and chronic inflammatory bowel disease. Ablation of EGC subpopulationsrevealed that the repair potential of intestinal stem cells (ISCs) is regulated bya specific subset of glial fibrillary acidic protein (GFAP)+ EGCs. Mechanistically, injury induces expansion of GFAP+ EGCs, which express several WNT ligands to promote LGR5+ ISC self-renewal. Our work reveals the dynamically regulated heterogeneity of EGCsas a key part of the intestinal stem cell niche in regeneration and disease.
DOI: 10.1016/j.stem.2021.10.004
Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00416-1
Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
官方网址：https://www.cell.com/cell-stem-cell/home
投稿链接：https://www.editorialmanager.com/cell-stem-cell/default.aspx
本期文章：《细胞—干细胞》：Online/在线发表
