德国杜塞尔多夫大学Jay Gopalakrishnan研究小组发现，人脑器官可组装出功能整合的双侧视泡。2021年8月17日，《细胞—干细胞》杂志在线发表了这项成果。
使用诱导多能干细胞（iPSC）衍生的人脑器官，研究人员试图简化复杂的过程，并展示前脑相关的双侧视泡的形成、细胞多样性和功能。在第30天左右，脑器官试图组装视泡，在60天内逐渐发展为可见的结构。这些含有视泡的脑类器官（OVB-organoid）构成了发育中的视泡的细胞成分，包括原始角膜上皮和晶状体细胞、视网膜色素上皮、视网膜祖细胞、轴突和电活性神经元网络。OVB-organoid还显示了突触素-1、CTIP阳性的髓鞘皮质神经元和小胶质细胞。
有趣的是，各种光强度可以触发OVB-organoid的光敏活动，而且光敏性可以在短暂的光漂白后重置。因此，脑器官具有自我组织前脑相关原始感觉结构的内在能力，并能在单一的器官内进行器官间的互动研究。
据了解，在胚胎发育过程中，视泡通过一个多步骤的器官形成过程从间脑发育出来。
附：英文原文
Title: Human brain organoids assemble functionally integrated bilateral optic vesicles
Author: Elke Gabriel, Walid Albanna, Giovanni Pasquini, Anand Ramani, Natasa Josipovic, Aruljothi Mariappan, Friedrich Schinzel, Celeste M. Karch, Guobin Bao, Marco Gottardo, Ata Alp Suren, Jürgen Hescheler, Kerstin Nagel-Wolfrum, Veronica Persico, Silvio O. Rizzoli, Janine Altmüller, Maria Giovanna Riparbelli, Giuliano Callaini, Olivier Goureau, Argyris Papantonis, Volker Busskamp, Toni Schneider, Jay Gopalakrishnan
Issue&Volume: 2021-08-17
Abstract: During embryogenesis, optic vesicles develop from the diencephalon via a multistep process of organogenesis. Using induced pluripotent stem cell (iPSC)-derived human brain organoids, we attempted to simplify the complexities and demonstrate formation of forebrain-associated bilateral optic vesicles, cellular diversity, and functionality. Around day 30, brain organoids attempt to assemble optic vesicles, which develop progressively as visible structures within 60 days. These optic vesicle-containing brain organoids (OVB-organoids) constitute a developing optic vesicle’s cellular components, including primitive corneal epithelial and lens-like cells, retinal pigment epithelia, retinal progenitor cells, axon-like projections, and electrically active neuronal networks. OVB-organoids also display synapsin-1, CTIP-positive myelinated cortical neurons, and microglia. Interestingly, various light intensities could trigger photosensitive activity of OVB-organoids, and light sensitivities could be reset after transient photobleaching. Thus, brain organoids have the intrinsic ability to self-organize forebrain-associated primitive sensory structures in a topographically restricted manner and can allow interorgan interaction studies within a single organoid.
DOI: 10.1016/j.stem.2021.07.010
Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00295-2
Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
官方网址：https://www.cell.com/cell-stem-cell/home
投稿链接：https://www.editorialmanager.com/cell-stem-cell/default.aspx
本期文章：《细胞—干细胞》：Online/在线发表
