比利时根特大学Pieter Mestdagh、Pavel Sumazin等研究人员合作拓展了人类非编码RNA的目录。相关论文于2021年6月17日在线发表于国际学术期刊《自然—生物技术》。
研究人员报道了一个更全面的人类转录组图谱，其中包括来自300种人类组织和细胞系的小RNA和多腺苷酸化RNA以及总RNA。研究人员报告了数千个以前未表征的RNA，这使得记录的非编码RNA（ncRNA）数量增加了大约8%。为了推断已知和新表征的ncRNA功能调节，利用总RNA测序中的前mRNA丰度估计，研究人员揭示了316个microRNA和3,310个长非编码RNA，并具有多种证据表明其在调节蛋白质编码基因和途径中的作用。
这项研究既完善又扩展了人类ncRNA及其调节相互作用的目录。所有数据、分析和结果均可在R2门户网站下载和查询，可作为未来探索RNA生物学和功能的基础。
据悉，现有的ncRNA纲要并不完整，部分原因是它们几乎完全来自小RNA和多腺苷酸化RNA。
附：英文原文
Title: The RNA Atlas expands the catalog of human non-coding RNAs
Author: Lucia Lorenzi, Hua-Sheng Chiu, Francisco Avila Cobos, Stephen Gross, Pieter-Jan Volders, Robrecht Cannoodt, Justine Nuytens, Katrien Vanderheyden, Jasper Anckaert, Steve Lefever, Aidan P. Tay, Eric J. de Bony, Wim Trypsteen, Fien Gysens, Marieke Vromman, Tine Goovaerts, Thomas Birkballe Hansen, Scott Kuersten, Nele Nijs, Tom Taghon, Karim Vermaelen, Ken R. Bracke, Yvan Saeys, Tim De Meyer, Nandan P. Deshpande, Govardhan Anande, Ting-Wen Chen, Marc R. Wilkins, Ashwin Unnikrishnan, Katleen De Preter, Jrgen Kjems, Jan Koster, Gary P. Schroth, Jo Vandesompele, Pavel Sumazin, Pieter Mestdagh
Issue&Volume: 2021-06-17
Abstract: Existing compendia of non-coding RNA (ncRNA) are incomplete, in part because they are derived almost exclusively from small and polyadenylated RNAs. Here we present a more comprehensive atlas of the human transcriptome, which includes small and polyA RNA as well as total RNA from 300 human tissues and cell lines. We report thousands of previously uncharacterized RNAs, increasing the number of documented ncRNAs by approximately 8%. To infer functional regulation by known and newly characterized ncRNAs, we exploited pre-mRNA abundance estimates from total RNA sequencing, revealing 316 microRNAs and 3,310 long non-coding RNAs with multiple lines of evidence for roles in regulating protein-coding genes and pathways. Our study both refines and expands the current catalog of human ncRNAs and their regulatory interactions. All data, analyses and results are available for download and interrogation in the R2 web portal, serving as a basis for future exploration of RNA biology and function.
DOI: 10.1038/s41587-021-00936-1
Source: https://www.nature.com/articles/s41587-021-00936-1
Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex
本期文章：《自然—生物技术》：Online/在线发表
