美国宾夕法尼亚大学Panteleimon Rompolas团队发现，Lgr6标记表皮干细胞，并在伤口再生中具有神经依赖性作用。这一研究成果于2021年6月7日在线发表在国际学术期刊《细胞—干细胞》上。
研究人员证明Lgr6标记了区域性限制的表皮干细胞群，这些干细胞与神经相互作用并专门从事伤口再生。白喉毒素介导的Lgr6干细胞敲除延迟了伤口愈合，并且皮肤去神经支配表型复制了这种效果。通过活体成像来捕获损伤后的干细胞动力学，研究人员表明Lgr6干细胞的伤口再生在神经丧失后减少。这会诱导其他干细胞群的募集，包括毛囊干细胞，其能够部分补偿伤口闭合。
单细胞谱系追踪和基因表达分析表明Lgr6干细胞的命运在失去它们的微环境后转向分化。因此，Lgr6表皮干细胞已经为损伤反应产生活化并与神经相互作用来调节其命运。
据悉，干细胞支持成年器官的终生维护，但它们在受伤期间的具体作用知之甚少。
附：英文原文
Title: Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization
Author: Sixia Huang, Paola Kuri, Yann Aubert, Megan Brewster, Ning Li, Olivia Farrelly, Gabriella Rice, Hyunjin Bae, Stephen Prouty, Tzvete Dentchev, Wenqin Luo, Brian C. Capell, Panteleimon Rompolas
Issue&Volume: 2021-06-07
Abstract: Stem cells support lifelong maintenance of adult organs, but their specific rolesduring injury are poorly understood. Here we demonstrate that Lgr6 marks a regionallyrestricted population of epidermal stem cells that interact with nerves and specializein wound re-epithelialization. Diphtheria toxin-mediated ablation of Lgr6 stem cellsdelays wound healing, and skin denervation phenocopies this effect. Using intravitalimaging to capture stem cell dynamics after injury, we show that wound re-epithelializationby Lgr6 stem cells is diminished following loss of nerves. This induces recruitmentof other stem cell populations, including hair follicle stem cells, which partiallycompensate to mediate wound closure. Single-cell lineage tracing and gene expressionanalysis reveal that the fate of Lgr6 stem cells is shifted toward differentiationfollowing loss of their niche. We conclude that Lgr6 epidermal stem cells are primedfor injury response and interact with nerves to regulate their fate.
DOI: 10.1016/j.stem.2021.05.007
Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00225-3
Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
官方网址：https://www.cell.com/cell-stem-cell/home
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本期文章：《细胞—干细胞》：Online/在线发表
