小柯机器人

法国南特大学Laurent David、Thomas Fréour等研究人员合作揭示人类胚胎着床前发育过程中谱系分化的动态改变。相关论文于2021年5月17日在线发表于国际学术期刊《细胞—干细胞》。

研究人员采用单细胞RNA测序（scRNA-seq）数据的伪时间分析来重建了早期小鼠和人类胚胎的发育。使用带注释的胚胎的延时成像，研究人员提供了整个人类发育过程中转录组学变化的集成、有序和连续的分析。研究人员揭示了人类滋养外胚层/内细胞团转录组在从B2到B3胚泡阶段的过渡，即在胚泡扩张之前发生了分支。

研究人员探索了命运标志物IFI16和GATA4的动力学，并显示它们分别在表皮细胞和原始内胚层命运建立后逐渐相互排斥。研究人员还提供了证据，表明NR2F2标记了滋养外胚层的成熟从极侧开始，然后在着床后扩散到所有细胞。这项研究查明了人类胚胎中谱系分化事件的准确时机，并确定了转录组学和细胞命运的标志。

据介绍，在人类着床前的发育过程中了解谱系分化是改进辅助生殖技术和干细胞研究的关键。

附：英文原文

Title: Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification

Author: Dimitri Meistermann, Alexandre Bruneau, Sophie Loubersac, Arnaud Reignier, Julie Firmin, Valentin Franois-Campion, Stéphanie Kilens, Yohann Lelièvre, Jenna Lammers, Magalie Feyeux, Phillipe Hulin, Steven Nedellec, Betty Bretin, Gal Castel, Nicolas Allègre, Simon Covin, Audrey Bihouée, Magali Soumillon, Tarjei Mikkelsen, Paul Barrière, Claire Chazaud, Joel Chappell, Vincent Pasque, Jérémie Bourdon, Thomas Fréour, Laurent David

Issue&Volume: 2021-05-17

Abstract: Understanding lineage specification during human pre-implantation development is agateway to improving assisted reproductive technologies and stem cell research. Herewe employ pseudotime analysis of single-cell RNA sequencing (scRNA-seq) data to reconstructearly mouse and human embryo development. Using time-lapse imaging of annotated embryos,we provide an integrated, ordered, and continuous analysis of transcriptomics changesthroughout human development. We reveal that human trophectoderm/inner cell mass transcriptomesdiverge at the transition from the B2 to the B3 blastocyst stage, just before blastocystexpansion. We explore the dynamics of the fate markers IFI16 and GATA4 and show thatthey gradually become mutually exclusive upon establishment of epiblast and primitiveendoderm fates, respectively. We also provide evidence that NR2F2 marks trophectodermmaturation, initiating from the polar side, and subsequently spreads to all cellsafter implantation. Our study pinpoints the precise timing of lineage specificationevents in the human embryo and identifies transcriptomics hallmarks and cell fatemarkers.

DOI: 10.1016/j.stem.2021.04.027

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00185-5

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