小柯机器人

美国宾夕法尼亚大学Panteleimon Rompolas研究团队通过双光子实时成像揭示角膜缘微环境中干细胞的区室化组织。相关论文于2021年5月12日在线发表在《细胞—干细胞》杂志上。

研究人员直接可视化了小鼠眼睛中的角膜缘，并鉴定了分散的干细胞微环境区室。通过记录单个干细胞及其后代的生命周期，研究人员直接分析了它们的命运，并表明它们在组织中的位置可以预测其分化状态。内角膜缘中的干细胞大部分经历对称分裂，并需要维持支持角膜稳态的瞬时祖细胞群。

使用原位光标记，研究人员捕获了它们的后代。缓慢细胞周期的干细胞在功能上是不同的，并且在稳态过程中表现出局部的克隆动力学，但是在损伤后可以促进角膜再生。这项研究证明了功能多样的干细胞群体如何实现区室化组织，从而促进成年器官的维持和再生。

据了解，成年器官中驻留干细胞的功能异质性尚不完全清楚。

附：英文原文

Title: Two-photon live imaging of single corneal stem cells reveals compartmentalized organization of the limbal niche

Author: Olivia Farrelly, Yoko Suzuki-Horiuchi, Megan Brewster, Paola Kuri, Sixia Huang, Gabriella Rice, Hyunjin Bae, Jianming Xu, Tzvete Dentchev, Vivian Lee, Panteleimon Rompolas

Issue&Volume: 2021-05-12

Abstract: The functional heterogeneity of resident stem cells that support adult organs is incompletelyunderstood. Here, we directly visualize the corneal limbus in the eyes of live miceand identify discrete stem cell niche compartments. By recording the life cycle ofindividual stem cells and their progeny, we directly analyze their fates and showthat their location within the tissue can predict their differentiation status. Stemcells in the inner limbus undergo mostly symmetric divisions and are required to sustainthe population of transient progenitors that support corneal homeostasis. Using in situ photolabeling, we captured their progeny exiting the niche before moving centripetallyin unison. The long-implicated slow-cycling stem cells are functionally distinct anddisplay local clonal dynamics during homeostasis but can contribute to corneal regenerationafter injury. This study demonstrates how the compartmentalized organization of functionallydiverse stem cell populations supports the maintenance and regeneration of an adultorgan.

DOI: 10.1016/j.stem.2021.02.022

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00073-4

Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
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